Air‐assisted in situ deep eutectic solvent decomposition followed by the solidification of floating organic droplets‐liquid‐liquid microextraction method for extraction of azole antifungal drugs in biological samples prior to high‐performance liquid chromatography

• Published in: Journal of separation science Vol. 45; no. 10; pp. 1757 - 1765
• Main Authors: Ezoddin, Maryam, Abdi, Khosrou, Behnamipour, Somaye, Javadi, Mahdiyeh H. S.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-05-2022
• Subjects: air‐assisted extraction; Analytical chemistry; Antifungal Agents; antifungal drugs; Azoles; Biological properties; Chromatography; Chromatography, High Pressure Liquid; Decomposition; Deep Eutectic Solvents; Dispersion; Dodecanol; Droplets; Drugs; Eutectic decomposition; Freezing; Fungicides; in situ deep eutectic solvent decomposition; Limit of Detection; Liquid chromatography; Liquid Phase Microextraction - methods; Melting points; Room temperature; Solidification; Solvents; Solvents - chemistry; Tetrabutylammonium bromide; in situ deep eutectic solvent decomposition; air-assisted extraction; antifungal drugs; solidification
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.202100965

A free dispersive method, air‐assisted in situ deep eutectic solvent decomposition followed by the solidification of floating organic droplets liquid‐liquid microextraction was indicated in this study. This technique was utilized to simultaneously ascertain some azole antifungal drugs prior to high‐performance liquid chromatography. In this research, a quasi‐hydrophobic deep eutectic solvent was formed from tetrabutylammonium bromide and 1‐dodecanol as an organic solvent at a 1:2 molar ratio. The synthesized deep decomposition in the sample solution caused in situ dispersion of extraction solvent and analytes. Air‐assisted enhanced a dispersion condition in the sample solution. 1‐Dodecanol as a green option was replaced with typical extraction solvents providing the advantages of a suitable freezing point near room temperature and low density. The effect of important analytical parameters on the extraction recovery of analytes was assessed. Under these optimal conditions, the limits of detection and the limits of quantitation determined were in the range of 0.5–2.8 and 1.5–9 μg/L, for water, urine and plasma samples, respectively. The intra‐day and inter‐day relative standard deviations (n = 5) were calculated to be 2.9–4.6 and 4.2–8.9%, respectively. The results represented the effectiveness of the developed method for the extraction and determination of analytes in biological samples.