Impact of air‐frying on the plasmalogens deterioration and oxidation in oyster revealed by mild acid hydrolysis and HILIC‐MS‐based lipidomics analysis

• Published in: Electrophoresis Vol. 42; no. 16; pp. 1552 - 1559
• Main Authors: Lu, Weibo, Zhang, Min, Zhang, Ting, Wang, Qingchen, Wang, Jie, Song, Gongshuai, Wang, Honghai, Feng, Junli, Shen, Qing
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-08-2021
• Subjects: Air‐frying; Animals; Frying; Hydrolysis; Hydrophilic interaction chromatography‐mass spectrometry; Lipidomics; Lipids; Ostreidae; Oxidation; Oyster; Phospholipids; Plasmalogens; Principal components analysis; Statistical analysis; Tandem Mass Spectrometry; Vinyl ethers; Lipidomics; Hydrophilic interaction chromatography-mass spectrometry; Air-frying; Oyster; Plasmalogens
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.202100106

Oyster is rich in plasmalogens that are ether phospholipids with biological functions to human body. Air‐frying is a popular technique for preparing delicious oyster but makes the plasmalogens vulnerable to oxidation. In this study, the effect of air‐frying processing on plasmalogens oxidation was studied by lipidomic approach. Plasmalogens were always mixed with normal phospholipids, thus the lipid extract was treated with mild acid hydrolysis to rapidly degrade plasmalogens owing to the acid lability of vinyl ether linkage at sn‐1 position. After hydrophilic interaction chromatography MS/MS analysis, there were three plasmalogen classes, plasmanylcholine, plasmanylethanolamine, and plasmanylinositol, completely separated, and each plasmalogen molecular species was identified and quantified. It indicated that the content of plasmalogens underwent an obvious decrease during the air‐frying process. To weaken such effect, the influence of air‐frying temperature was further inspected by multivariate statistical analyses. The main variables, including the ions of m/z 756.4927, 784.5486, 828.5812, etc., were revealed by unsupervised principle component analysis, supervised orthogonal partial least‐square analysis, and variable importance in projection plot. As a conclusion, air‐frying has health benefits in reducing fat content but destructive to plasmalogens, thus interventions are recommended to prevent the degradation of plasmalogens.