An improved in vitro immunization procedure for the production of monoclonal antibodies against neural and other antigens

A simplified in vitro immunization procedure has been developed for the production of monoclonal antibodies against a variety of neural antigens. The procedure, which can be applied to both soluble and membrane-bound antigens, involves the addition of an adjuvant peptide, N-acetylmuramyl-L-alanyl-D-...

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Bibliographic Details
Published in:	Brain research Vol. 291; no. 1; p. 193
Main Author:	Boss, B D
Format:	Journal Article
Language:	English
Published:	Netherlands 16-01-1984
Subjects:	Acetylmuramyl-Alanyl-Isoglutamine - administration & dosage Animals Animals, Newborn - immunology Antibodies, Monoclonal - biosynthesis Cattle Cells, Cultured Glutamate Decarboxylase - immunology Hippocampus - immunology Hybridomas - immunology Nervous System - immunology Rats
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Summary:	A simplified in vitro immunization procedure has been developed for the production of monoclonal antibodies against a variety of neural antigens. The procedure, which can be applied to both soluble and membrane-bound antigens, involves the addition of an adjuvant peptide, N-acetylmuramyl-L-alanyl-D-isoglutamine, and has been found to improve all fusion parameters.
ISSN:	0006-8993
DOI:	10.1016/0006-8993(84)90671-1