Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by Entrapment in Polyvinyl Alcohol and Its Application to Neo-Fructooligosaccharides Production

• Published in: Catalysts Vol. 8; no. 5; p. 201
• Main Authors: Noa Míguez, María Gimeno-Pérez, David Fernández-Polo, Fadia V. Cervantes, Antonio O. Ballesteros, María Fernández-Lobato, María H. Ribeiro, Francisco J. Plou
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-05-2018
• Subjects: bioreactors; enzyme entrapment; fructooligosaccharides; glycosidases; hydrogels; immobilization; neokestose; prebiotics
• ISSN: 2073-4344
• DOI: 10.3390/catal8050201

The β-fructofuranosidase (Xd-INV) from the basidiomycota yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) is unique in its ability to synthesize neo- fructooligosaccharides (neo-FOS). In order to facilitate its industrial application, the recombinant enzyme expressed in Pichia pastoris (pXd-INV) was immobilized by entrapment in polyvinyl alcohol (PVA) hydrogels. The encapsulation efficiency exceeded 80%. The PVA lenticular particles of immobilized pXd-INV were stable up to approximately 40 °C. Using 600 g/L sucrose, the immobilized biocatalyst synthesized 18.9% (w/w) FOS (59.1 g/L of neokestose, 30.2 g/L of 1-kestose, 11.6 g/L of neonystose and 12.6 g/L of blastose). The operational stability of PVA-immobilized biocatalyst was assayed in a batch reactor at 30 °C. The enzyme preserved its initial activity during at least 7 cycles of 26 h.