cytochrome oxidase subunit III gene in sunflower mitochondria is cotranscribed with an open reading frame conserved in higher plants

• Published in: Current genetics Vol. 18; no. 4; pp. 355 - 363
• Main Authors: Quagliariello, C, Saiardi, A, Gallerani, R
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-11-1990; Berlin
• Subjects: Amino Acid Sequence; amino acid sequences; Base Sequence; Biological and medical sciences; Chromosome Mapping; Codon; cytochrome-c oxidase; DNA, Mitochondrial - analysis; Electron Transport Complex IV - genetics; Fundamental and applied biological sciences. Psychology; genes; Genes, Plant; Genes. Genome; Helianthus - genetics; Helianthus annuus; Introns; mitochondria; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequences; Open Reading Frames; RNA Processing, Post-Transcriptional; Sequence Homology, Nucleic Acid; transcription (genetics); Transcription, Genetic; Cytochrome c oxidase; Nucleotide sequence; Transcription; Enzyme; Homology; Mitochondrial DNA; Compositae; Gene expression; Secondary structure; Mitochondria; Conserved sequence; Helianthus annuus; Dicotyledones; Angiospermae; Spermatophyta; Molecular cloning
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00318217

The gene encoding subunit III of cytochrome oxidase (COXIII) has been identified in the sunflower mitochondrial genome. The COXIII coding region is located 570 bp downstream of a 477 bp open reading frame (ORFB). Sequence comparisons and hybridization experiments show that ORFB sequences are conserved in other plant mitochondrial genomes. Nucleotide and amino acid sequence comparisons suggest that RNA editing is required in sunflower mitochondria to synthesize a functional COXIII polypeptide.