Pressure tolerance of Artemia cysts compressed in water medium

• Published in: High pressure research Vol. 39; no. 2; pp. 293 - 300
• Main Authors: Hazael, Rachael, Matsuda, Shinsuke, Mori, Yoshihisa, Fitzmaurice, Brianna C., Appleby-Thomas, Gareth, Painter, Jonathan D., Meersman, Filip, Richaud, Myriam, Galas, Simon, Saini, Naurang L., Ono, Fumihisa, McMillan, Paul F.
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 03-04-2019; Taylor & Francis Ltd
• Subjects: Artemia; Artemia salina; Biomolecules; Crystallization; Cysts; Eggs; Embryos; hatchability; high pressure tolerance; Ice formation; ice VI phase; Life Sciences; osmotic pressure; Survival; Trademarks; osmotic pressure; Artemia salina; ice VI phase; high pressure tolerance; cysts; hatchability
• ISSN: 0895-7959; 1477-2299
• DOI: 10.1080/08957959.2019.1575378

The high pressure tolerance of cysts of Artemia salina was investigated up to several GPa in water. No survival was observed after exposure to 1.0 GPa for 15 min. After exposure to 2.0 GPa for the same time duration, the hatching rate had recovered to 33%, but decreased to 8% following compression at 7.5 GPa. This contrasts with results using Fluorinert™ as the pressure-transmitting medium where 80-88% recovery was observed. The lower survival rate in water is accompanied by swelling of the eggs, indicating that liquid H
2
O close to the ice-VI crystallization pressure penetrated inside the eggs. This pressure exceeds the stability limit for proteins and other key biomolecules components within the embryos that could not be resuscitated. Rehydration takes several minutes and so was not completed for all samples compressed to higher pressures, prior to ice-VI formation, resulting in renewed survival. However H
2
O penetration inside the shell resulted in increased mortality.