Distribution and Identification of Ulva aragoensis (Ulvaceae, Chlorophyta), a Constituent Species of Green Tides in the Southern Yellow Sea, Based on Molecular Data

• Published in: Journal of marine science and engineering Vol. 10; no. 11; p. 1767
• Main Authors: Tong, Yichao, Xia, Lihua, Liu, Jinlin, Zhao, Shuang, Sun, Yuqing, Wu, Tingjian, Xia, Zhangyi, Li, Shuang, Cao, Jiaxing, Zhang, Jianheng
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-11-2022
• Subjects: Algae; Chemical analysis; Community composition; Constituents; DNA barcoding; DNA sequencing; Gene sequencing; green tide; Green tides; haplotype; Haplotypes; Laboratories; micro-propagule; Morphology; Nucleotide sequence; Propagules; RbcL gene; Ribosomal DNA; Seawater; Southern Yellow Sea; Species composition; TrnL gene; Ulva aragoensis; Ulva flexuosa; Water analysis; Yellow Sea; China
• ISSN: 2077-1312; 2077-1312
• DOI: 10.3390/jmse10111767

Green tides are a serious global ecological disaster; the largest occur in the Southern Yellow Sea (SYS). Early-stage green tides in the SYS are composed of four species (Ulva prolifera, Ulva flexuosa, Ulva linza, and Ulva compressa). We found that U. aragoensis is a constituent species of green tides in the SYS based on molecular data. Furthermore, this study re-evaluated the proportion of U. aragoensis in green tides and found that it was more prevalent in micro-propagules cultured from surface seawater during an early-stage green tide in 2021. The internal transcribed spacers, tufA, 18S, rbcL, large subunit, psbA, and rps2-trnL gene sequences were compared; the tufA and rbcL gene sequences were the most suitable DNA barcodes for distinguishing U. aragoensis. A haplotype analysis of the sequences of floating U. aragoensis and its micro-propagules was performed to study the correlation between green tide macroalgae and micro-propagules; close haplotype similarities occurred between them. This study further clarified the species composition of SYS green tides and provided a reference for assessing the relationship between micro-propagules and green tide macroalgae.