Middle cerebral artery occlusion in the rat causes a biphasic production of immunoreactive interleukin-1 β in the cerebral cortex

• Published in: Neuroscience letters Vol. 249; no. 2; pp. 177 - 179
• Main Authors: Hillhouse, E.W, Kida, S, Iannotti, F
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 19-06-1998; Elsevier
• Subjects: Biological and medical sciences; Cerebral ischaemia; Interleukin- β; Medical sciences; Neurology; Neuronal death; Vascular diseases and vascular malformations of the nervous system; Neuronal death; Cerebral ischaemia; Interleukin- β; Nervous system diseases; Cerebral cortex; Rat; Cytokine; Rodentia; Cardiovascular disease; Interleukin 1β; Cerebral disorder; Vascular disease; Vertebrata; Experimental disease; Mammalia; Ischemia; Cell death; Animal; Central nervous system disease; Cerebrovascular disease; Brain (vertebrata)
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(98)00392-9

Interleukin-1
β (IL-1
β) has been implicated in the sequence of events leading to neuronal cell death. We have used an ultrasensitive, highly specific immunometric assay for rat IL-1
β to determine the pattern of protein expression in the rat cerebral cortex following middle cerebral artery occlusion. In the ipsilateral cerebral cortex there was a biphasic pattern of production with a rapid early phase of IL-1
β expression followed by a delayed phase at 4 h which was sustained for up to 72 h. There was a similar but smaller expression of IL-1
β in the contralateral cortex. Sham operated animals showed no IL-1
β expression. These results provide further evidence to show that IL-1
β is expressed in the rat cerebral cortex following cerebral ischaemia.