PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the cichlid fish in Lake Victoria, Haplochromis chilotes

• Published in: Molecular ecology resources Vol. 8; no. 2; pp. 428 - 430
• Main Authors: MAEDA, K., TAKESHIMA, H., MIZOIRI, S., OKADA, N., NISHIDA, M., TACHIDA, H.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-03-2008
• Subjects: cichlid; haplochromine; Lake Victoria; microsatellite
• ISSN: 1755-098X; 1755-0998
• DOI: 10.1111/j.1471-8286.2007.01981.x

Twelve short tandem repeat markers were successfully isolated from a cichlid, Haplochromis chilotes, in Lake Victoria, and characterized in Haplochromis pyrrhocephalus. The microsatellite regions of these markers were found to have between two and 48 alleles with heterozygosity ranging from 0.07 to 0.97. No loci showed significant departures from the Hardy–Weinberg or linkage equilibrium after the Bonferroni correction (P > 0.05). Cross‐species amplification in other cichlids of Lake Victoria, Haplochromis laparogramma, Lithochromis rubripinnis, L. rufus and Haplochromis sp. ‘rockkribensis’, was successful.