MMP-2, MMP-9 and TIMP-2 gene polymorphisms in Chinese patients with generalized aggressive periodontitis

• Published in: Journal of clinical periodontology Vol. 34; no. 5; pp. 384 - 389
• Main Authors: Chen, Dong, Wang, Qi, Ma, Zhi-Wei, Chen, Fa-Ming, Chen, Yue, Xie, Guang-Yuan, Wang, Qin-Tao, Wu, Zhi-Fen
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-05-2007
• Subjects: Adult; aggressive periodontitis; Asian Continental Ancestry Group - genetics; Case-Control Studies; China; Dentistry; Female; Genetic Predisposition to Disease; Humans; Male; matrix metalloproteinase; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase Inhibitors; Mouth Mucosa - enzymology; Periodontitis - enzymology; Periodontitis - genetics; Polymerase Chain Reaction; polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; tissue inhibitor of metalloproteinase; Tissue Inhibitor of Metalloproteinase-2 - genetics; China
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2007.01071.x

Background: Aggressive periodontitis (AgP) has a genetic basis. It has been reported that the functional gene polymorphisms of matrix metalloproteinase (MMP)‐2, MMP‐9 and tissue inhibitor of metalloproteinase‐2 (TIMP‐2) alter their expressions in transcriptional level and they are involved in the tissue destruction of periodontitis. The study was carried out to analyse the association of functional polymorphisms in MMP‐2, MMP‐9 and TIMP‐2 with generalized AgP (G‐AgP) in a Chinese population.
Material and Methods: The study population consisted of 79 Chinese patients with G‐AgP and 128 healthy controls. DNA was obtained from oral mucosa swab samples. MMP‐2 genotypes were determined by PCR‐based denaturing high‐performance liquid chromatography analysis while MMP‐9 and TIMP‐2 genotypes were identified by a PCR‐based restriction fragment length polymorphism. χ2 test after Yates' correction was used to investigate the possible association of the genotypes with the G‐AgP.
Results: Although gene polymorphisms for MMP‐2 and MMP‐9 did not show any association with the G‐AgP, the analysis of the TIMP‐2 −418G to C gene polymorphism revealed significant differences between the patients and controls. Compared with controls, a significant increasing trend of TIMP‐2 −418C carrier in the G‐AgP patients occurred (p=0.013).
Conclusion: It is suggested that the TIMP2 −418G to C gene polymorphism is associated with G‐AgP in the Chinese subjects.