Synthesis of S-[ 13N]nitrosoglutathione ( 13N-GSNO) as a new potential PET imaging agent

• Published in: Applied radiation and isotopes Vol. 67; no. 1; pp. 95 - 99
• Main Authors: Llop, Jordi, Gómez-Vallejo, Vanessa, Bosque, Marta, Quincoces, Gemma, Peñuelas, Iván
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 2009
• Subjects: 13N; GSNO; Isotope Labeling - methods; Nitric oxide; Nitrogen Radioisotopes; NO donor; PET; Positron-Emission Tomography - methods; Radiopharmaceuticals - chemical synthesis; S-Nitrosoglutathione - chemical synthesis; S-nitrosothiols; Solutions; Water; S-nitrosothiols; 13N; GSNO; Nitric oxide; PET; NO donor
• ISSN: 0969-8043; 1872-9800
• DOI: 10.1016/j.apradiso.2008.09.014

In the present paper, a fast and reproducible method for the synthesis of S-[
13N]nitrosoglutathione is reported for the first time. The labeling strategy is based on the production of [
13N]NO
3
− via the
16O(
p,
α)
13N nuclear reaction in water, as opposed to the standardized production of [
13N]NH
4
+ in 2
mM aqueous ethanol. Following the reduction of [
13N]NO
3
− to [
13N]NO
2
−, the reaction with glutathione in the presence of hydrochloric acid led to the desired radiotracer with a good radiochemical yield (24.2±2.0% end of synthesis,
n=5) in a short production time (3
min from the end of bombardment).