Chromosomal mapping of the uracil permease gene of Saccharomyces cerevisiae

• Published in: Current genetics Vol. 11; no. 2; pp. 93 - 96
• Main Authors: Weber, E, Jund, R, Chevallier, M.R
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-01-1986; Berlin
• Subjects: Biological and medical sciences; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; DNA Restriction Enzymes; enzymes; Fundamental and applied biological sciences. Psychology; Genes; Genes, Fungal; Genetic Linkage; Genetics of eukaryotes. Biological and molecular evolution; Genotype; Membrane Transport Proteins - genetics; Nucleotide Transport Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Thallophyta, bryophyta; Vegetals; Fungi; Genetic mapping; Yeast; Gene; Membrane transport; Chromosome; Ascomycetes; Saccharomyces cerevisiae; Uracil; Thallophyta
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00378199

The gene FUR4, coding for the uracil permease in Saccharomyces cerevisiae, was mapped on chromosome II, at a distance of 7.8 cM from the centromere on the right arm of the chromosome. In a first step, we used the chromosome loss mapping method developed by Falco and Botstein (1983) to determine on which chromosome the gene mapped. After the observation that FUR4 was closely linked to GAL10, one of the three genes forming the gal cluster (Bassel and Mortimer 1971), we could determine precisely the position of the gene on chromosome II.