The inhibitory effects of camptothecin (CPT) and its derivatives on the substrate uptakes mediated by human solute carrier transporters (SLCs)

• Published in: Xenobiotica Vol. 46; no. 9; pp. 831 - 840
• Main Authors: Zheng, Jian, Chan, Ting, Zhu, Ling, Yan, Xiufeng, Cao, Zhisong, Wang, Yang, Zhou, Fanfan
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-09-2016
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Camptothecin; Camptothecin - pharmacology; Drug Interactions; drug-drug interaction; HEK293 Cells; Humans; organic anion transporters; Organic Anion Transporters - metabolism; organic anion transporting polypeptides; organic cation transporters; Camptothecin; organic cation transporters; drug–drug interaction; organic anion transporting polypeptides; organic anion transporters
• ISSN: 0049-8254; 1366-5928
• DOI: 10.3109/00498254.2015.1129080

1. Camptothecin (CPT) and its derivatives are potent candidate compounds in treating cancers. However, their clinical applications are largely restricted by severe toxicities.
2. The solute carrier transporters (SLCs), particularly the organic anion transporting polypeptides and organic anion/cation transporters (OATs/OCTs) are widely expressed in human key organs and responsible for the cellular influx of many substances including endogenous substrates and many clinically important drugs. Drug-drug interactions through SLCs often result in unsatisfied therapeutic outcomes and/or unexpected toxicities.
3. This study investigated the inhibitory effects of CPT and its eight derivatives on the cellular uptake of specific substrates mediated by the essential SLCs in over-expressing Human embryonic kidney 293 cells.
4. Our data revealed that CPT, 10-hydroxycamptothecin (HCPT), 10-methoxycamptothecin (MCPT) and 9-nitrocamptothecin (9NC) significantly inhibit the uptake activity of OAT3. 9NC also inhibited the substrate transport mediated by OAT1. The substrate uptakes of OAT1, OCTN1 and OCTN2 were significantly decreased in the presence of CZ112, while CPT-11 potently down-regulated the transport activity of OCT1 and OCT3.
5. In summary, our study demonstrated that CPT and its eight derivatives selectively inhibit the substrate uptakes mediated by the essential SLCs. This information contributes to understanding the localized toxicity of CPTs and provides novel molecular targets for the therapeutic optimization of CPTs in the future.