Polypeptide N-acetylgalactosaminyltransferase-15 regulates adipogenesis in human SGBS cells

• Published in: Scientific reports Vol. 14; no. 1; pp. 20049 - 12
• Main Authors: Takahashi, Asuka, Koike, Ryo, Watanabe, Shota, Kuribayashi, Kyoko, Wabitsch, Martin, Miyamoto, Masahiko, Komuro, Akihiko, Seki, Mineaki, Nashimoto, Masayuki, Shimizu-Ibuka, Akiko, Yamashita, Kikuji, Iwata, Takeo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29-08-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 3T3-L1 Cells; 631/136; 692/163/2743/393; Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis; Adipogenesis - genetics; Animals; CCAAT-enhancer binding protein; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; Cell Line; DNA microarrays; GALNT15; Gene expression; Humanities and Social Sciences; Humans; Leptin; Leptin - genetics; Leptin - metabolism; Mice; Molecular modelling; multidisciplinary; N-acetylgalactosaminyltransferase; N-Acetylgalactosaminyltransferases - genetics; N-Acetylgalactosaminyltransferases - metabolism; Peroxisome proliferator-activated receptors; Polypeptide N-acetylgalactosaminyltransferase; Polypeptides; PPAR gamma - genetics; PPAR gamma - metabolism; Preadipocytes; Science; Science (multidisciplinary); Transcription factors; Preadipocytes; Adipocytes; Polypeptide; acetylgalactosaminyltransferase; Adipogenesis; Polypeptide N-acetylgalactosaminyltransferase; GALNT15
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-70930-5

Adipogenesis involves intricate molecular mechanisms regulated by various transcription factors and signaling pathways. In this study, we aimed to identify factors specifically induced during adipogenesis in the human preadipocyte cell line, SGBS, but not in the mouse preadipocyte cell line, 3T3-L1. Microarray analysis revealed distinct gene expression profiles, with 1460 genes induced in SGBS cells and 1297 genes induced in 3T3-L1 cells during adipogenesis, with only 297 genes commonly induced. Among the genes uniquely induced in SGBS cells, we focused on
GALNT15
, which encodes polypeptide
N
-acetylgalactosaminyltransferase-15. Its expression increased transiently during adipogenesis in SGBS cells but remained low in 3T3-L1 cells. Overexpression of
GALNT15
increased mRNA levels of CCAAT-enhancer binding protein (C/EBPα) and leptin but had no significant impact on adipogenesis in SGBS cells. Conversely, knockdown of
GALNT15
suppressed mRNA expression of adipocyte marker genes, reduced lipid accumulation, and decreased the percentage of cells with oil droplets. The induction of C/EBPα and peroxisome proliferator-activated receptor γ during adipogenesis was promoted or suppressed in SGBS cells subjected to overexpression or knockdown of
GALNT1
5, respectively
.
These data suggest that polypeptide
N
-acetylgalactosaminyltransferase-15 is a novel regulatory molecule that enhances adipogenesis in SGBS cells.