Cloning of a DNA repeat element from horse: DNA sequence and chromosomal localization

A DNA repeat element, revealed initially by digestion of horse DNA with TaqI, was cloned and characterized by Southern and in situ hybridization studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiteration of a unit repeat of 21-22 bp, and produced multilocus DNA fingerprin...

Full description

Saved in:

Bibliographic Details
Published in:	Genome Vol. 38; no. 6; p. 1132
Main Authors:	Broad, T E, Forrest, J W, Lewis, P E, Pearce, P D, Phua, S H, Pugh, P A, Stewart-Scott, I A
Format:	Journal Article
Language:	English
Published:	Canada 01-12-1995
Subjects:	Animals Base Sequence Centromere Chromosome Mapping Cloning, Molecular Consensus Sequence - genetics DNA Fingerprinting Fathers Female Horses - genetics In Situ Hybridization Male Molecular Sequence Data Repetitive Sequences, Nucleic Acid - genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A DNA repeat element, revealed initially by digestion of horse DNA with TaqI, was cloned and characterized by Southern and in situ hybridization studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiteration of a unit repeat of 21-22 bp, and produced multilocus DNA fingerprinting profiles that were useful for parentage analysis in horses. The tandem repeat element was shown by in situ hybridization to be localized in the centromeres of the acrocentric but not metacentric classes of horse chromosomes.
ISSN:	0831-2796
DOI:	10.1139/g95-150