CLASSIFICATION OF PSEUDOMONAS AERUGINOSA O ANTIGENS BY IMMUNOELECTROPHORESIS

• Published in: Journal of medical microbiology Vol. 8; no. 2; pp. 225 - 233
• Main Authors: Lanyi, B, Adam, Maria M, Szentmihalyi, Anna
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-05-1975
• Subjects: Agglutination; Antigen-Antibody Reactions; Antigens, Bacterial - analysis; Antigens, Bacterial - classification; Cross Reactions; Densitometry; Immunoelectrophoresis; Lipopolysaccharides - analysis; Pseudomonas aeruginosa - classification; Pseudomonas aeruginosa - immunology; Sodium Hydroxide; Trichloroacetic Acid
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/00222615-8-2-225

National Institute of Public Health, H-1966 Budapest, POB 64, Hungary
Received July 17, 1974
Accepted October 15, 1974
Heated saline extracts of 89 strains, and (1) supernates of phenol-water extracts (L 1 fractions), (2) purified lipopolysaccharide, (3) trichloracetic-acid (TCA) extracts, and (4) sodium-hydroxide extracts of 23 strains representing all Pseudomonas aeruginosa O antigens were subjected to electrophoresis. Precipitation lines obtained with homologous and heterologous antisera were evaluated by electrodensitometric measurement. The characteristics of the immunoelectrophoretic groups established were as follows. Group I : two lines running at different rates towards the anode; three subgroups on the basis of the behaviour of alkali-treated antigens. Group II : triple line at the starting well, alkali sensitive. Group III : triple line at the starting well, alkali resistant; two subgroups according to reactivity or non-reactivity of L 1 fractions. Group IV : triple line on the cathode side, alkali resistant, L 1 fraction non-reactive. Group V : single line on the anode side, alkali sensitive, L 1 fraction and TCA extract non-reactive. O antigens identified by agglutination corresponded closely with the immunoelectrophoretic pattern: strains with identical O antigens or sharing major somatic components fell, with one exception, into the same immunoelectrophoretic group.