Development of a polyclonal antibody-based indirect competitive ELISA for the determination of tetrodotoxins in marine ribbon worms (NEMERTEA) and its comparison with high performance liquid chromatography-tandem mass spectrometry

• Published in: Toxicon (Oxford) Vol. 176; pp. 30 - 33
• Main Authors: Vlasenko, A.E., Kuznetsov, V.G., Petrova, I. Yu, Magarlamov, T. Yu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-03-2020
• Subjects: Antibodies; ELISA; Guanidinium toxins; HPLC-MS/MS; Tetrodotoxin; Tetrodotoxin analogues; Tetrodotoxin; Antibodies; HPLC-MS/MS; Guanidinium toxins; Tetrodotoxin analogues; ELISA
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2020.01.009

Tetrodotoxin (TTX) is a potent neurotoxin frequently occurring in marine organisms along with its numerous analogues. To determine the total TTX content, we developed an enzyme-linked immunosorbent assay (ELISA) technique utilizing polyclonal antibodies against TTX. The technique was tested using extracts of marine worms of the phylum Nemertea and confirmed by HPLC-MS/MS. It proved to be suitable for a preliminary assessment of the toxicity of marine organisms.
•ELISA technique for quantification of summed TTXs concentration has been developed.•Antibodies against TTXs were obtained by immunization with PHA-TTX conjugate.•The reliability of developed method was confirmed by its comparison with HPLC-MS/MS.