Acute-phase protein-like properties of endoplasmic reticulum aminopeptidase 1

• Published in: Journal of biochemistry (Tokyo) Vol. 165; no. 2; pp. 159 - 165
• Main Authors: Goto, Yoshikuni, Nakamura, Takahiro J, Ogawa, Kenji, Hattori, Akira, Tsujimoto, Masafumi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-02-2019
• Subjects: Acute-Phase Proteins - metabolism; Aminopeptidases - blood; Aminopeptidases - deficiency; Aminopeptidases - metabolism; Animals; Inflammation - chemically induced; Inflammation - metabolism; Leucyl Aminopeptidase - metabolism; Lipopolysaccharides - administration & dosage; Mice; Mice, Inbred C57BL; Mice, Knockout; Minor Histocompatibility Antigens - blood; Minor Histocompatibility Antigens - metabolism; Nitric Oxide - biosynthesis; nitric oxide; lipopolysaccharide; amino acid; acute phase; aminopeptidase
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/jb/mvy090

Abstract
Endoplasmic reticulum aminopeptidase 1 (ERAP1) is a multi-functional enzyme. In this study, we analysed its role in lipopolysaccharide-induced inflammatory response in wild-type and ERAP1-knockout mice. Following lipopolysaccharide injection, ERAP1 was secreted into the blood, increasing leucine aminopeptidase activity and NO synthesis therein. Among the amino acids tested, arginine concentration was significantly increased in wild-type mice compared to ERAP1-knockout mice. These results suggest that ERAP1 behaves similar to acute-phase proteins, which are secreted into the blood in response to infectious/inflammatory stimuli and are involved in enhancing NO synthesis as a host defense mechanism.