Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria

• Published in: Microbial pathogenesis Vol. 117; pp. 68 - 72
• Main Authors: Saravanan, Muthupandian, Arokiyaraj, Selvaraj, Lakshmi, Thangavelu, Pugazhendhi, Arivalagan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2018
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Bacteria - drug effects; Basidiomycota - metabolism; Fibroblasts - drug effects; Human pathogen; Humans; Klebsiella pneumoniae - drug effects; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Particle Size; Phenerochaete chrysosporium; Pseudomonas aeruginosa - drug effects; Silver - chemistry; Silver nanoparticles; Staphylococcus aureus - drug effects; Staphylococcus epidermidis - drug effects; Antibacterial activity; Phenerochaete chrysosporium; Human pathogen; Silver nanoparticles
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2018.02.008

The present study elucidates an eco-friendly method for synthesizing silver nanoparticles using Phenerochaete chrysosporium (MTCC-787), its bactericidal and cytotoxic effect were studied. The formation of nanoparticles was evidenced by color change and UV–Vis spectroscopy. Atomic Force Microscope and Transmission electron microscope, showed spherical and oval shapes particles in the sizes ranging between 34 and 90 nm. The biosynthesised silver nanoparticles showed significant antibacterial activity against Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Staphylococcus epidermidis at a high dose. Further, the nanoparticles observed to be non-toxic at 12.5 μg/ml towards fibroblast cells.
•Extracellular synthesis of Ag-NPs from the Phenerochaete chrysosporium (MTCC-787).•Ag-NPs were characterized by UV-Spec, FTIR, AFM & TEM.•Ag-NPs showed antibacterial potency against P. aeruginosa, K. pneumoniae, S. aureus and S. epidermidis.