Antifungal activities of diphenyl diselenide alone and in combination with fluconazole or amphotericin B against Candida glabrata

• Published in: Mycopathologia (1975) Vol. 176; no. 1-2; pp. 165 - 169
• Main Authors: Denardi, Laura Bedin, Mario, Débora Alves Nunes, de Loreto, Erico Silva, Nogueira, Cristina Wayne, Santurio, Janio Morais, Alves, Sydney Hartz
• Format: Journal Article
• Language: English
• Published: Netherlands Springer 01-08-2013
• Subjects: Amphotericin B; Amphotericin B - pharmacology; Antifungal Agents - pharmacology; Antiparasitic agents; Benzene Derivatives - pharmacology; Candida glabrata; Candida glabrata - drug effects; Drug Interactions; Fluconazole; Fluconazole - pharmacology; Microbial Sensitivity Tests; Organoselenium Compounds - pharmacology
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1007/s11046-013-9672-x

Here, we evaluated combinations of diphenyl diselenide [(PhSe)2] with fluconazole and amphotericin B in a checkerboard assay against clinical Candida glabrata strains. Minimal inhibitory concentration (geometric mean) ranged from 0.25 to >64 (5.16 μg/mL) for (PhSe)2, 1 to 32 (5.04 μg/mL) for fluconazole and 0.06 to 0.5 (0.18 μg/mL) for amphotericin B. Synergistic (76.66 %) and indifferent (23.34 %) interactions were observed for (PhSe)2 + amphotericin B combination. (PhSe)2 + fluconazole combination demonstrated indifferent (50 %) and antagonistic (40 %) interactions, whereas synergistic interactions were observed in 10 % of the isolates. New experimental in vivo protocols are necessary and will promote a better understanding of the antimicrobial activity of (PhSe)2 against C. glabrata and its use as an adjuvant therapy with antifungal agents.