Quantification of Glucose, fructose and 1,5-Anhydroglucitol in plasma of diabetic patients by ultra performance liquid chromatography tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1200; p. 123277
• Main Authors: Li, Mengna, Yan, Dewen, Hao, Mingyu, Huang, Xiaohong, Xu, Yun, Li, Weifeng, Liu, Wenlan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-2022
• Subjects: 1,5-anhydroglucitol; Diabetes mellitus; Fructose; Glucose; UPLC-MS/MS; UPLC-MS/MS; Glucose; Diabetes mellitus; Fructose; 1,5-anhydroglucitol
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2022.123277

•Development of a UPLC-MS/MS method for the determination of glucose, fructose, and 1,5-AG in plasma.•Accurate and precise measurement of glucose, fructose, and 1,5-AG in the millimole and micromole range.•Rapid in use, simple sample pretreatment, and short chromatographic runtime of 10 min.•Clinical application is demonstrated in a number of T2DM in different pathophysiological conditions.
Type 2 diabetes mellitus (T2DM), a worldwide disease that affects the quality of human life and social development. Glucose, fructose and 1,5-anhydroglucitol are closely related to diabetes mellitus. However, few methods have been reported to achieve these three carbohydrates in the blood simultaneously. In this study, a UPLC-MS/MS method allowing to quantify glucose, fructose, and 1,5-anhydroglucitol simultaneously in human plasma was developed. The analysis was performed by UPLC-MS/MS system with HILIC column. This new method provided satisfactory results in terms of calibration curves with good linearity (R2 > 0.99) over 3 order of magnitude range, precision (coefficient of variation of intra-day and inter-day: 0.72–10.23% and 2.21–13.8%), accuracy (results of intra-day and inter-day: 97–113%, 100–107%), matrix effects (87–109%), recovery (93–119%), carry-over (0.004–0.014%), as well as stability (0.04–6.9%) within the acceptance criteria. The reproducible, precise and accurate method with suitable dynamic ranges was successfully applied to the analysis of glucose, fructose and 1,5-anhydroglucitol in T2DM under different pathophysiological conditions.