Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum

• Published in: Carbohydrate polymers Vol. 123; pp. 350 - 358
• Main Authors: Maity, Prasenjit, Sen, Ipsita K., Maji, Praloy K., Paloi, Soumitra, Devi, K. Sanjana P., Acharya, Krishnendu, Maiti, Tapas K., Islam, Syed S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 05-06-2015
• Subjects: Agaricales; Antioxidant properties; Antioxidants - chemistry; Antioxidants - isolation & purification; Basidiomycota - chemistry; Basidiomycota - metabolism; beta-Glucans - chemistry; beta-Glucans - isolation & purification; beta-Glucans - pharmacology; Carbohydrate Sequence; Entoloma lividoalbum; Fruiting Bodies, Fungal - chemistry; Fruiting Bodies, Fungal - metabolism; Gas Chromatography-Mass Spectrometry; Immunostimulation; Macrophages - cytology; Macrophages - drug effects; Macrophages - immunology; Magnetic Resonance Spectroscopy; Molecular Sequence Data; NMR studies; Superoxides - chemistry; β-D-Glucan; β-D-Glucan; Antioxidant properties; Immunostimulation; Entoloma lividoalbum; NMR studies
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2015.01.051

•A water soluble β-D-glucan was isolated from the mushroom Entoloma lividoalbum.•Structure of the glucan was established by chemical and 1D/2D NMR experiments.•This molecule showed immunoenhancing and antioxidant activities.
A water soluble β-glucan having molecular weight ∼2×105Da was isolated from hot water extract of the fruit bodies of an edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička. This polysaccharide (ELPS) contains (1→3,6)-β-D-Glcp, (1→3)-β-D-Glcp, (1→6)-β-D-Glcp, and terminal β-D-Glcp moieties in a molar ratio of nearly 1:1:3:1. Chemical and spectroscopic analysis showed that the backbone of glucan consists of three (1→6)-β-D-glucopyranosyl and two (1→3)-β-D-glucopyranosyl residues, out of which one (1→3)-β-D-glucopyranosyl moiety was branched at O-6 with a terminal β-D-glucopyranosyl residue. This β-glucan exhibited macrophage, splenocyte, and thymocyte stimulations. It possesses promising antioxidant activities as evidenced from its hydroxyl and superoxide radical scavenging activities and reducing properties.