Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum

• Published in: International journal of systematic and evolutionary microbiology Vol. 52; no. 1; pp. 157 - 164
• Main Authors: Menes, R. J, Muxi, L
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-01-2002; Society for General Microbiology
• Subjects: Anaerobaculum mobile; Bacteriology; Biological and medical sciences; Crotonates - metabolism; DNA, Ribosomal - analysis; Electron Transport; Fermentation; Fundamental and applied biological sciences. Psychology; Gram-Negative Anaerobic Bacteria - classification; Gram-Negative Anaerobic Bacteria - genetics; Gram-Negative Anaerobic Bacteria - isolation & purification; Gram-Negative Anaerobic Bacteria - physiology; Industrial Waste; Microbiology; Molecular Sequence Data; Peptides - metabolism; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Temperature; Water Microbiology; Phenotype; Description; Taxonomy; Clostridiales; Bacteria; Thermophily; New species; Atoll lagoon; Waste water
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/00207713-52-1-157

R. J. Menes and L. Muxi
Catedra de Microbiologia, Facultad de Quimica y Facultad de Ciencias, Universidad de la Republica, CC 1157, Montevideo, Uruguay
A novel anaerobic, moderately thermophilic, peptide-fermenting bacterium,
strain NGA(T), was isolated from an anaerobic wool-scouring wastewater
treatment lagoon. The cells were Gram-negative, straight rods of 0.5--1.0 x
2.0--4.0 microm, motile by means of a single flagellum. The DNA G+C content
was 51.5 mol%. The optimum pH and temperature range for growth were
6.6--7.3 and 55--60 degrees C, respectively. The optimum NaCl concentration
was 0.08 g l(-1). The bacterium fermented organic acids (malate, tartrate,
pyruvate, glycerol and fumarate), a few carbohydrates (starch, glucose,
fructose and gluconate), Casamino acids, tryptone and yeast extract.
Carbohydrates and organic acids were converted to acetate, hydrogen and
CO(2). The bacterium oxidized leucine to isovalerate with crotonate as an
electron acceptor, but not in co-culture with Methanothermobacter
thermoautotrophicus DSM 3720(T). Thiosulfate, sulfur and cystine were
reduced to sulfide and crotonate was reduced to butyrate with glucose and
tryptone-yeast extract as electron donors. Phylogenetic analysis of the 16S
rRNA gene indicated that strain NGA(T) was related to Anaerobaculum
thermoterrenum (98% similarity ), the only described species of the genus.
The DNA--DNA hybridization value for strain NGA(T) and A. thermoterrenum
ACM 5076(T) was 40.8%. On the basis of these results, strain NGA(T) is
proposed as a novel species of the genus Anaerobaculum, namely
Anaerobaculum mobile sp. nov. The type strain is NGA(T) (=DSM 13181(T)=ATCC
BAA-54(T)).