A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection

• Published in: Talanta (Oxford) Vol. 144; pp. 466 - 473
• Main Authors: Chammem, H., Hafaid, I., Bohli, N., Garcia, A., Meilhac, O., Abdelghani, A., Mora, L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-11-2015
• Subjects: AFM; Antibodies, Immobilized - chemistry; Antibodies, Immobilized - immunology; Antigens - immunology; Apolipoprotein A-I - immunology; Bacterial Proteins - chemistry; Bacterial Proteins - immunology; Biosensing Techniques; Contact angle; Dielectric Spectroscopy; Electrochemical Techniques; Electrodes; Gold - chemistry; HDL; Humans; Immunoglobulin G - chemistry; Immunoglobulin G - immunology; Impedance spectroscopy; Lipoproteins, HDL - analysis; Lipoproteins, HDL - blood; Lipoproteins, HDL - immunology; Microscopy, Atomic Force; Protein G; Serum Albumin, Bovine - chemistry; AFM; HDL; Contact angle; Impedance spectroscopy; Protein G
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2015.06.009

In this work, two biosensors were developed for the detection of High-Density Lipoproteins (HDL) particles, which are biomarkers inversely correlated with cardiovascular risk and which represent therapeutic targets for atherosclerosis. The electrochemical properties of the grafted antibody on interdigitated gold electrode were achieved by Impedance Spectroscopy (IS). The used deposition method was based on oriented antibody Anti-ApoA1 with an intermediate thin layer of protein G. The developed biosensor was able to detect both native plasma HDL and reconstituted HDL (rHDL) particles respectively with the detection limit of 50ng/mL and 1ng/mL, respectively. Dynamic contact angle and atomic force microscopy were used. The developed biosensors are able to differentiate the HDL particles according to their differences in size and interactions with the immobilized antibody.
•We develop two biosensors for plasma and reconstituted HDL detection, respectively.•It is shown that impedance spectroscopy can differentiate HDL and rHDL.•Contact angle measurements and AFM confirm impedance spectroscopy results.