Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects

• Published in: Carbohydrate polymers Vol. 262; p. 117925
• Main Authors: Ning, Xin, Liu, Ying, Jia, Mengdi, Wang, Qidi, Sun, Ziyan, Ji, Li, Mayo, Kevin H., Zhou, Yifa, Sun, Lin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15-06-2021
• Subjects: Antioxidant activities; Pectin; Radix Sophorae Tonkinensis; Structural characterization; Radix Sophorae Tonkinensis; Pectin; Antioxidant activities; Structural characterization
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.117925

•Two pectic polysaccharides were purified from Radix Sophorae Tonkinensis.•They both contained HG, RG-I and RG-II domains with different mass ratios.•RG-I domains in them varied in Mw, degree of branching and side chain structures.•They had different antioxidant activities toward scavenging of different radicals.•HG domain rich in GalA might be the key factor for antioxidant activity of pectin.
Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities. Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively. The RG-I domains were further purified and characterized. Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains. WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains. Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.