Polygonum odoratum leaf extract attenuates oxidative stress and cell death of Raw 264.7 cells exposed to low dose ionizing radiation

• Published in: Journal of food biochemistry Vol. 46; no. 4; pp. e13909 - n/a
• Main Authors: Kawvised, Supannika, Prabsattroo, Thawatchai, Munkong, Waranon, Pattum, Panuwat, Iamsaard, Sittichai, Boonsirichai, Kanokporn, Uttayarat, Pimporn, Maikaeo, Lamai, Sudchai, Waraporn, Kirisattayakul, Woranan
• Format: Journal Article
• Language: English
• Published: United States 01-04-2022
• Subjects: Animals; antioxidant; Antioxidants - metabolism; Antioxidants - pharmacology; Cell Death; low dose ionizing radiation; Mice; Oxidative Stress; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polygonum - chemistry; Polygonum odoratum; Radiation, Ionizing; RAW 264.7 Cells; Superoxide Dismutase - metabolism; low dose ionizing radiation; antioxidant; oxidative stress; Polygonum odoratum
• ISSN: 0145-8884; 1745-4514
• DOI: 10.1111/jfbc.13909

This study aims to investigate the effect of Polygonum odoratum leaf extract (POE) on oxidative stress markers and cell death induced by low dose ionizing radiation (LDIR) in Raw 264.7 cells. The biological activities, chromatographic fingerprint, and cytotoxicity of POE were investigated. To determine the radioprotective effect of POE, Raw 264.7 cells were incubated with POE for 1 hr prior to 100 mGy x‐irradiation. The cell viability, oxidative stress damage marker (malondialdehyde level; MDA), and endogenous antioxidant markers (superoxide dismutase: SOD, catalase: CAT, and glutathione peroxidase: GSH‐Px) were also determined. The results showed that POE contained 8 essential substances and exhibited a potent antioxidant without any cytotoxicity. It was found that POE significantly decreased the MDA level and activated cell viability, SOD, CAT, and GSH‐Px activities. The results from this study indicate that POE is a potent antioxidant, which can be developed as a radioprotector for diagnostic procedures.
Practical applications
Polygonum odoratum leaf extract (POE) is a potent antioxidant that attenuates oxidative stress and cell death induced by low dose ionizing radiation (LDIR). POE might protect against cell damage from LDIR, particularly in diagnostic radiology procedures. Therefore, the development of functional food containing POE might be beneficial for patients who plan to undergo the diagnostic radiology procedure. The functional food containing POE might prevent stochastic and deterministic effects for these patients.
Polygonum odoratum leaf extract (POE) is contained 8 essential substances and exhibited a potent antioxidant without any cytotoxicity.
POE significantly decreased the MDA level and activated cell viability, SOD, CAT, and GSH‐Px activities.
POE is a potent antioxidant which can be developed as a radioprotector for diagnostic procedures.