Pseudonocardia babensis sp. nov., isolated from plant litter

• Published in: International journal of systematic and evolutionary microbiology Vol. 60; no. Pt 10; pp. 2336 - 2340
• Main Authors: SAKIYAMA, Yayoi, THAO, Nguyen K. N, VINH, Hoang V, GIANG, Nguyen M, MIYADOH, Shinji, HOP, Duong V, ANDO, Katsuhiko
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01-10-2010
• Subjects: Actinomycetales - classification; Actinomycetales - cytology; Actinomycetales - genetics; Actinomycetales - isolation & purification; Arabinose - analysis; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Cell Wall - chemistry; Cluster Analysis; Diaminopimelic Acid - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Galactose - analysis; Microbiology; Microscopy, Electron, Scanning; Molecular Sequence Data; Phospholipids - analysis; Phylogeny; Plants - microbiology; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Spores, Bacterial - cytology; Systematics; Vietnam; Vietnam; Pseudonocardia; Litter; Taxonomy; Bacteria; Actinomycetes; New species; Pseudonocardiaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.018127-0

A novel actinomycete, designated strain VN05A0561(T), was isolated from plant litter collected at Ba Be National Park, Vietnam. The substrate mycelia and spore chains fragmented in a manner similar to nocardioform actinomycetes; the spores had smooth surfaces and were rod-shaped. Strain VN05A0561(T) had the following chemotaxonomic characteristics: meso-diaminopimelic acid in the cell-wall peptidoglycan, arabinose and galactose as characteristic sugars, MK-8(H₄) as the major isoprenoid quinone, phosphatidylcholine as the diagnostic phospholipid and iso-C₁₆:₀ as the major cellular fatty acid. Strain VN05A0561(T) shared low levels of 16S rRNA gene sequence similarity (< 97 %) with the type strains of recognized species of the genus Pseudonocardia and could be differentiated from its closest phylogenetic relatives based on phenotypic characteristics. These results suggested that strain VN05A0561(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia babensis sp. nov. is proposed. The type strain is VN05A0561(T) (=VTCC-A-1757(T)=NBRC 105793(T)).