Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

• Published in: Applied sciences Vol. 10; no. 3; p. 738
• Main Authors: Sotnikov, Dmitriy V., Barshevskaya, Lyubov V., Zherdev, Anatoly V., Eskendirova, Saule Z., Mukanov, Kassym K., Mukantayev, Kanatbek K., Ramankulov, Yerlan M., Dzantiev, Boris B.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-02-2020
• Subjects: Antibodies; Antigens; Aqueous solutions; Binding sites; Brucellosis; Cattle; Conjugates; cow diseases; functionalized nanoparticles; Gold; Immunoglobulins; Laboratories; lateral flow tests; Markers; Nanoparticles; Pathogens; Proteins; Quantum dots; rapid tests; signal enhancement; veterinary diagnostics; St Louis Missouri; United States > US; Russia
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app10030738

In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjugate and by more than nine times compared with the system using the GNP conjugate.