Antibiotic resistance genes prediction via whole genome sequence analysis of Stenotrophomonas maltophilia

• Published in: Journal of infection and public health Vol. 17; no. 2; pp. 236 - 244
• Main Authors: Shahid, Sara, Abid, Rameesha, Ajmal, Wajya, Almuqbil, Mansour, Almadani, Moneer E, Khan, Yasir, Ansari, Adnan Ahmad, Rani, Rehana, Alshehri, Ahmed, Alghamdi, Adel, Asdaq, Syed Mohammad Basheeruddin, Ghazanfar, Shakira
• Format: Journal Article
• Language: English
• Published: England Elsevier 01-02-2024
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotic Resistance Genes; Drug Resistance, Bacterial - genetics; Gram-Negative Bacterial Infections - microbiology; Humans; Next Generation Sequencing (NGS); Phylogeny; Sequence Analysis; Stenotrophomonas maltophilia; Stenotrophomonas maltophilia - genetics; Whole Genome Sequencing (WGS); Stenotrophomonas maltophilia; Next Generation Sequencing (NGS); Whole Genome Sequencing (WGS); Antibiotic Resistance Genes
• ISSN: 1876-0341; 1876-035X
• DOI: 10.1016/j.jiph.2023.12.010

Stenotrophomonas maltophilia (S. maltophilia) is the first dominant ubiquitous bacterial species identified from the genus Stenotrophomonas in 1943 from a human source. S. maltophilia clinical strains are resistance to several therapies, this study is designed to investigate the whole genome sequence and antimicrobial resistance genes prediction in Stenotrophomonas maltophilia (S. maltophilia) SARC-5 and SARC-6 strains, isolated from the nasopharyngeal samples of an immunocompromised patient.
These bacterial strains were obtained from Pakistan Institute of Medical Sciences (PIMS) Hospital, Pakistan. The bacterial genome was sequenced using a whole-genome shotgun via a commercial service that used an NGS (Next Generation Sequencing) technology called as Illumina Hiseq 2000 system for genomic sequencing. Moreover, detailed in-silico analyses were done to predict the presence of antibiotic resistance genes in S. maltophilia.
Results showed that S. maltophilia is a rare gram negative, rod-shaped, non sporulating bacteria. The genome assembly results in 24 contigs (>500 bp) having a size of 4668,850 bp with 65.8% GC contents. Phylogenetic analysis showed that SARC-5 and SARC-6 were closely related to S. maltophilia B111, S. maltophilia BAB-5317, S. maltophilia AHL, S. maltophilia BAB-5307, S. maltophilia RD-AZPVI_04, S. maltophilia JFZ2, S. maltophilia RD_MAAMIB_06 and lastly with S. maltophilia sp ROi7. Moreover, the whole genome sequence analysis of both SARC-5 and SARC-6 revealed the presence of four resistance genes adeF, qacG, adeF, and smeR.
Our study confirmed that S. maltophilia SARC-5 and SARC-6 are one of the leading causes of nosocomial infection which carry multiple antibiotic resistance genes.