Effects of Bovine Serum Albumin on Boar Sperm Quality During Liquid Storage at 17°C

• Published in: Reproduction in domestic animals Vol. 50; no. 2; pp. 263 - 269
• Main Authors: Zhang, X-G, Yan, G-J, Hong, J-Y, Su, Z-Z, Yang, G-S, Li, Q-W, Hu, J-H
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-04-2015
• Subjects: Animal reproduction; Animals; Antioxidants; Bovine serum albumin; Cell Membrane - drug effects; Cell Membrane - physiology; Dilution; Hogs; Integrity; Male; Malondialdehyde; Motility; Oxidative stress; Preservation; Semen; Semen Preservation - methods; Semen Preservation - veterinary; Serum albumin; Serum Albumin, Bovine - pharmacology; Sperm; Spermatozoa - cytology; Spermatozoa - drug effects; Spermatozoa - physiology; Statistical analysis; Statistical methods; Storage; Survival; Swine - physiology; Temperature
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.12481

Contents
This study aimed to investigate the effects of bovine serum albumin (BSA) on boar sperm quality during liquid storage at 17°C. Boar semen samples were collected and diluted with Modena containing different concentrations (0, 1, 2, 3, 4, 5 and 6 g/l) of BSA, and sperm motility, plasma membrane integrity, acrosome integrity, total antioxidative capacity (T‐AOC) activity and malondialdehyde (MDA) content were measured and analysed. The results showed that Modena supplemented with 3, 4 and 5 g/l BSA could improve boar sperm motility, effective survival time and plasma membrane integrity (p < 0.05), decrease MDA content (p < 0.05), while no statistical difference was observed for sperm acrosome integrity and T‐AOC activity among these three groups (p > 0.05). The semen sample diluted with Modena containing 4 g/l BSA could achieve optimum effect, and sperm survival time was 7.5 days. After 7 days preservation, sperm motility, plasma membrane integrity and acrosome integrity were 54%, 49% and 78%, respectively. T‐AOC activity and MDA content were 1.03 U/ml and 17.5 nmol/ml, respectively. In conclusion, Modena supplemented with BSA reduced the oxidative stress and improved the sperm quality of boar semen during liquid storage at 17°C, and 4 g/l BSA was the optimum concentration. Further studies are required to obtain more concrete results on the determination of antioxidant capacities of BSA in liquid preserved boar semen.