No significant effect of orally administered chemokine receptor 1 antagonist on intercellular adhesion molecule-3 expression in relapsing—remitting multiple sclerosis patients

• Published in: Multiple sclerosis Vol. 16; no. 3; pp. 366 - 369
• Main Authors: Reuβ, R., Schreiber, V., Klein, A., Infante-Duarte, C., Filippi, M., Pabst, W., Pohl, C., Oschmann, P.
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-03-2010; Sage Publications Ltd
• Subjects: Administration, Oral; Antigens, CD - blood; Cell Adhesion Molecules - blood; Double-Blind Method; Flow Cytometry; Germany; Humans; Immunologic Factors - administration & dosage; Intercellular Adhesion Molecule-1 - blood; Italy; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - immunology; Lipopolysaccharide Receptors - blood; Multiple Sclerosis, Relapsing-Remitting - drug therapy; Multiple Sclerosis, Relapsing-Remitting - immunology; Phenylurea Compounds - administration & dosage; Piperidines - administration & dosage; Receptors, CCR1 - antagonists & inhibitors; Time Factors; Treatment Outcome; intercellular adhesion molecule-3; flow cytometry; receptor cross-talk; chemokine receptor 1 antagonist; MRI; multiple sclerosis
• ISSN: 1352-4585; 1477-0970
• DOI: 10.1177/1352458509358188

We investigated the expression of intercellular adhesion molecules ICAM-1 and ICAM-3 on peripheral blood mononuclear cells in a subgroup of 34 patients with relapsing-remitting multiple sclerosis who were treated orally with the chemokine receptor 1 antagonist BX 471 in a 16-week, randomised, double-blind, placebo-controlled phase II study. ICAM-1 and ICAM-3 expression was measured by flow cytometry at different time points during and after therapy and compared using multivariate analysis of variance and non-parametric Mann Whitney test. ICAM-3 expression on CD14 + peripheral blood mononuclear cells was increased in the verum group under therapy, but did not differ significantly between the verum and placebo groups. Most likely, this trend represents a small epiphenomenon only mediated by receptor cross-talk and feedback mechanisms.