Inhibitory effect of anthocyanin-rich black soybean testa (Glycine max (L.) Merr.) on the inflammation-induced adipogenesis in a DIO mouse model

• Published in: Journal of functional foods Vol. 14; pp. 623 - 633
• Main Authors: Kim, So Young, Wi, Hae-Ri, Choi, Seojin, Ha, Tae Joung, Lee, Byong Won, Lee, Myoungsook
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-04-2015; Elsevier
• Subjects: Adipogenesis; Anthocyanin; Black soybean testa; Inflammation; Lipogenesis; Lipolysis; C/EBPβ; C57BL/6N; FFA; C/EBPα; BBC; C/EBPγ; ALT; MDA; RT-PCR; Lipogenesis; HSL; Anthocyanin; IL-1; H&E; TBARS; IL-6; IL-10; PPARγ; Lipolysis; SDS-PAGE; MAG; D3G; GAPDH; PCR; ELISA; MCP-1; P3G; ATGL; SREBP-1; LPL; Black soybean testa; O; AMPKα; ANOVA; LPS/IFN-γ; HPLC; Adipogenesis; ACC; AST; CON; Inflammation; DAG; DIO; TC; p-AMPKα; TG; TNF-α; C3G; FAS; HF
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2015.02.030

•We found there are three major anthocyanins in the ethanol extract from seed coat of black soybean (BB): C3G, D3G, and P3G.•BB significantly decreases food intake and fat accumulation in DIO C57BL/6N mouse model.•We demonstrated the successful lipolysis-promoting effects of BB; the upregulation of HSL, LPL and p-AMPK expression.•Our result confirms the anti-inflammatory effect of BB; the downregulation of TNF-α and IL-6 expression.
Black soybeans have been known as medicinal food with anti-obesity, anti-inflammation and anti-oxidative stress effects, and the testa of Cheongja #3 black soybeans is especially rich in anthocyanins. Forty-one male C57BL/6N mice were randomly assigned into four groups after 10-week-period of diet-induced obesity: a control group, a high-fat diet group, a high-fat diet group with the ethanol extract from black soybean testa (BBC), and a high-fat diet group with orlistat; BBC was orally administered for 12 weeks. BBC treatment significantly decreased food intake, fat accumulation, and the expression of lipogenesis genes (acetyl-CoA carboxylase and CCAAT-enhancer-binding protein alpha), and increased the levels of lipolysis proteins (lipoprotein lipase, hormone-sensitive lipase, and AMP-activated protein kinase) in mesenteric fat. BBC also showed successful anti-inflammatory effects through regulation of tumor necrosis factor-alpha, interleukin-6 and interleukin-10. These findings provide scientific support for using anthocyanin-rich black soybean as functional food in prevention of obesity and inflammation-induced diseases.