Production of the bioactive isoflavone O-desmethylangolensin by Enterococcus faecium INIA P553 with high efficiency

• Published in: Journal of functional foods Vol. 40; pp. 180 - 186
• Main Authors: Gaya, Pilar, Peirotén, Ángela, Álvarez, Inmaculada, Medina, Margarita, Landete, José Mª.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-01-2018; Elsevier
• Subjects: Enterococcus faecium; Food; Isoflavones; O-desmethylangolensin; Probiotic; Probiotic; Enterococcus faecium; O-desmethylangolensin; Isoflavones; Food
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2017.11.008

•In vitro studies suggests that O-DMA has several cancer-related biological actions.•Enteroccus faecium INIA P553 has probiotic potential.•E. faecium INIA P553 produced O-DMA from dihydrodaidzein, daidzein, daidzin and formononetin,•E. faecium INIA P553 produced 6-hydroxy O-DMA from dihydrogenistein, genistein and genistin.
Healthy effect of isoflavones consumption is attributed to their bioactive metabolites such as O-desmethylangolensin (O-DMA) produced by intestinal bacteria. Evidence from in vitro studies suggests that O-DMA has several cancer-related biological actions. Enterococcus spp. are indigenous species of the human gastrointestinal tract. Significant attention has been focused on Enterococcus faecium strains with probiotic and /or technological interest in foods. In this work, we describe the production of the bioactive isoflavones O-DMA and 6-hydroxy O-DMA by the strain E. faecium INIA P553. O-DMA was produced from dihydrodaidzein, daidzein, daidzin and formononetin, and 6-hydroxy O-DMA from dihydrogenistein, genistein and genistin with high efficiency. To our knowledge, it is the first bacterium with probiotic potential capable of producing O-DMA and 6-hydroxy O-DMA. Therefore, this strain shows potential applications in biotechnology in functional foods and as probiotic.