Topic use of Annona crassiflora Mart. contributes to wound healing due to the antioxidant and proliferative effects of fibroblasts

• Published in: Injury Vol. 53; no. 3; pp. 844 - 857
• Main Authors: de Moura, Francyelle Borges Rosa, Ferreira, Bruno Antonio, Muniz, Elusca Helena, Justino, Allisson Benatti, Silva, Ana Gabriela, Santos, Rinara de Almeida, Gomide, José Augusto Leoncio, Ribeiro, Rosy Iara Maciel de Azambuja, Ribeiro, Daniele Lisboa, Araújo, Fernanda de Assis, Espindola, Foued Salmen, Tomiosso, Tatiana Carla
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01-03-2022
• Subjects: Animals; Annona; Annonaceae; Antioxidants - pharmacology; Bioactive compounds; Cerrado; Fibroblasts; Mice; Phenolic compounds; Plant Extracts - pharmacology; Skin; Wound Healing; Skin; Annonaceae; Cerrado; Phenolic compounds; Bioactive compounds
• ISSN: 0020-1383; 1879-0267
• DOI: 10.1016/j.injury.2021.12.007

•Phenolic compounds obtained from A. crassilora fruit peel (PCAc) have healing effect.•PCAc has proliferative effect on fibroblasts in vitro test.•Wounds treated with PCAs showed reduced inflammation.•PCAc protects injured tissue from oxidative damage.•Treatment of wounds with PCAc has no pro-angiogenic effect.
Annona crassiflora Mart. is a species native to the Cerrado biome, whose fruit is known as araticum or marolo. Plant parts are widely used in folk medicine to treat inflammation and pain associated with rheumatism, wounds, venereal diseases, snakebites, and microbial infections. Thus, we investigated a fraction rich in phenolic compounds (PCAc) obtained from the crude extract of the peel of these fruits on non-cytotoxic, anti-inflammatory, antioxidant, and collagen biosynthesis properties in the healing of wounds induced on the back of BALB/c mice. For the control group, the induced wounds were not treated and for the others, wounds were treated topically with vehicle or vehicle plus PCAc. Both fractions contained in PCAc demonstrated effective protection on fibroblasts. We highlight the effect of the ethyl acetate fraction which, in addition to the protective effect, has a proliferative activity on these cells. In addition, PCAc caused improvement in healing after 7 days of treatment and in the longest period of treatment with PCAc (7, 14, and 21 days) there was a greater contraction of the wound, accompanied by resolution of the inflammatory process, antioxidant defense, increasing collagen synthesis, and modulation of metalloproteinases. PCAc demonstrated better re-epithelialization and organization of the dermis at the end of treatment. The changes promoted by the phenolic compounds of A. crassiflora were important in the healing process, especially in activities related to inflammation, oxidative stress, and fibrogenesis.