Synthesis and biological evaluation of flavone-8-acrylamide derivatives as potential multi-target-directed anti Alzheimer agents and investigation of binding mechanism with acetylcholinesterase

• Published in: Bioorganic chemistry Vol. 88; p. 102960
• Main Authors: Shaik, Jeelan Basha, Yeggoni, Daniel Pushparaju, Kandrakonda, Yelamanda Rao, Penumala, Mohan, Zinka, Raveendra Babu, Kotapati, Kasi Viswanath, Darla, Mark Manidhar, Ampasala, Dinakara Rao, Subramanyam, Rajagopal, Amooru, Damu Gangaiah
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2019
• Subjects: Alzheimer’s disease; Cyanoacetamide; Interaction with AChE; Methoxyflavones; MTDLs; MTDLs; Cyanoacetamide; Alzheimer’s disease; Methoxyflavones; Interaction with AChE
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/j.bioorg.2019.102960

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•A series of 7-methoxyflavone and cyanoacetamides hybrids (8a–q) were synthesized.•Synthetic analogs were evaluated for their AChE, BuChE, ABTS inhibitory, neuroprotective and Aβ modulating abilities.•Most compounds had excellent anti AChE, ABTS inhibitory, neuroprotective and Aβ modulating abilities.•Compounds 8a, 8d, 8e, 8h and 8i might serve as potential lead in search of innovative MTDLs for AD.•The molecular interaction studies clearly suggesting that AChE-flavone complex is more stable.
In a search for novel multifunctional anti-Alzheimer agents, a congeneric set of seventeen flavone-8-acrylamide derivatives (8a─q) were synthesized and evaluated for their cholinesterase inhibitory, antioxidant, neuroprotective and modulation of Aβ aggregation activities. The target compounds showed effective and selective inhibitory activity against the AChE over BuChE. In addition, the target compounds also showed moderate anti-oxidant activity and strong neuroprotective capacities, and accelerated dosage-dependently the Aβ aggregation. Also, we presented here a complete study on the interaction of 8a, 8d, 8e, 8h and 8i with AChE. Through fluorescence emission studies, the binding sites number found to be 1, binding constants were calculated as 2.04 × 104, 2.22 × 104, 1.18 × 104, 9.8 × 103 and 3.2 × 104 M−1 and free energy change as −5.83, −5.91, −5.51, −5.41 and −6.12 kcal M−1 at 25 °C which were well agreed with the computational calculations indicating a strong binding affinity of flavones and AChE. Furthermore, the CD studies revealed that the secondary structure of AChE became partly unfolded upon binding with 8a, 8d, 8e, 8h and 8i.