Rosuvastatin based novel 3-substituted isocoumarins / 3-alkylidenephthalides: Ultrasound assisted synthesis and identification of new anticancer agents

• Published in: European journal of medicinal chemistry Vol. 201; p. 112335
• Main Authors: Kumar, Jetta Sandeep, Thirupataiah, B., Medishetti, Raghavender, Ray, Aramita, Bele, Shilpak Dilip, Hossain, Kazi Amirul, Reddy, Gangireddy Sujeevan, Edwin, Rebecca Kristina, Joseph, Alex, Kumar, Nitesh, Shenoy, Gautham G., Rao, C. Mallikarjuna, Pal, Manojit
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01-09-2020
• Subjects: Animals; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - pharmacology; Antineoplastic Agents - toxicity; Benzofurans - chemical synthesis; Benzofurans - pharmacology; Benzofurans - toxicity; Cancer; Cell Line, Tumor; Drug Screening Assays, Antitumor; Embryo, Nonmammalian - drug effects; Humans; Isocoumarin; Isocoumarins - chemical synthesis; Isocoumarins - pharmacology; Isocoumarins - toxicity; Molecular Structure; Phthalide; Rosuvastatin; Rosuvastatin Calcium - analogs & derivatives; Rosuvastatin Calcium - pharmacology; Rosuvastatin Calcium - toxicity; Structure-Activity Relationship; Ultrasonic Waves; Zebrafish; Zebrafish; Isocoumarin; Phthalide; Rosuvastatin; Cancer
• ISSN: 0223-5234; 1768-3254
• DOI: 10.1016/j.ejmech.2020.112335

A new class of 3-substituted isocoumarin/3-alkylidenephthalide based novel small molecules derived from rosuvastatin were designed and synthesized via the ultrasound assisted Cu-mediated coupling-cyclization in a single pot with remarkable regioselectivity. The phthalides were generally obtained at lower temperature whereas the use of elevated temperature afforded isocoumarins. Two compounds e.g. 3n and 4d showed promising cytotoxic effects when tested against HCT 116, HepG2 and PA-1 cell lines at 10 μM. Indeed, 4d was found to be a potent cytotoxic agent (IC50 ∼ 0.76–4.51 μM). Both 3n and 4d were tested for their effects on PANC-1 cells. Considerable decrease in p-Akt substrates shown by 4d and 3n at 50 μM (western blot analysis) indicated their ability to inhibit p-Akt signal transduction pathway and arresting growth of PANC-1 cells in vitro. This was further supported by the cytotoxic effect of 4d on PANC-1 cells (MTT assay) that was better than rosuvastatin. While none of 3n and 4d showed any significant effect on non-cancerous HEK cell line (indicating their potential selectivity towards cancer cells) these compounds were further evaluated for their toxicities in Zebrafish embryo. The NOAEL (No Observed Adverse Effect Level) for teratogenicity, hepatotoxicity and cardiotoxicity was found to be 100 μM for both compound. Thus, 4d as a novel and potent but safer cytotoxic agent with potential to treat colorectal/ovarian and pancreatic cancer is of further medicinal interest.
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