Physiological adaptation and spectral annotation of Arsenic and Cadmium heavy metal-resistant and susceptible strain Pseudomonas taiwanensis

• Published in: Environmental pollution (1987) Vol. 251; pp. 555 - 563
• Main Authors: Satapute, Praveen, Paidi, Murali Krishna, Kurjogi, Mahantesh, Jogaiah, Sudisha
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-08-2019
• Subjects: Heavy metals; Intracellular structural organization; Protein level; Pseudomonas taiwanensis; Stress enzymes; Pseudomonas taiwanensis; Protein level; Intracellular structural organization; Heavy metals; Stress enzymes
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2019.05.054

In the present study, the 16S-rRNA sequencing of heavy metal-resistant and susceptible bacterial strains isolated from the industrial and agriculture soil showed resemblance with Pseudomonas taiwanensis. Based on the growth rate, two bacterial strains SJPS_KUD54 and KUD-MBBT4 exhibited 10 ppm tolerance to Arsenic and Cadmium. These two heavy metals caused, a significant increase in stress enzymes like superoxide dismutase, catalase and glutathione S-transferase activities in SJPS_KUD54 when compared to KUD-MBBT4. Following heavy metal treatment, the atomic-force-microscopy observations showed no change in the cell-wall of SJPS_KUD54, whereas the cell-wall of KUD-MBBT4 got ruptured. Moreover, the protein-profile of SJPS_KUD54 treated with heavy metals exhibited varied patterns in comparison with untreated control. In addition, the accumulation of hydroxyl, thiol and amides were found in the SJPS_KUD54 relative to its control. Furthermore, the resistant SJPS_KUD54 strain showed a remarkable bioaccumulation properties to both Arsenic and Cadmium. Thus, it is inferred that the growth rate, stress enzymes and functional-groups play a significant role in the physiological-adaption of SJPS_KUD54 during stress conditions, which is positively involved in the prevention or repair mechanism for reducing the risks caused by heavy metal stress.
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•Bacterium possessing bioabsorption of heavy metals was isolated.•The impact of heavy metals on microbial growth and enzyme activities was studied.•SJPS_KUD54 strain exhibited physiological adaption to stress conditions.•IR functional group confirms the resistant strain SJPS_KUD54 to metal tolerance.•Resistant strain can be utilized for bioremediation of heavy metals and waste management.