The rDNA intergenic spacer region nucleotide sequence of Hordeum bulbosum L

• Published in: Plant molecular biology Vol. 15; no. 4; pp. 661 - 663
• Main Authors: Procunier, J.D. (Guelph Univ., Guelph, Ont. (Canada). Dept. of Crop Science), Kasha, K.J
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-10-1990
• Subjects: ADN; ARN RIBOSOMAL; ARN RIBOSOMIAL; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Hordeum - genetics; HORDEUM BULBOSUM; Introns; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; RIBOSOMAL RNA; RNA, Ribosomal, 18S - genetics; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Monocotyledones; Nucleotide sequence; Gramineae; Repeated sequence; Angiospermae; Ribosomal DNA; Gene organization; Spacer DNA; Regulatory sequence; Spermatophyta; Hordeum bulbosum
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00017841

A ribosomal RNA gene (rDNA) intergenic spacer unit was cloned from H. bulbosum , a species related to barley, and the molecular organization of the region flanking the 5' end of the 18S gene was determined. The intergenic spacer region is of particular interest since it contains DNA sequences for the promoter region, enhances of transcription, transcription termination, rRNA processing and possibly for rDNA replication and unequal crossing-over.