C6 glioma-derived microvesicles stimulate the proliferative and metastatic gene expression of normal astrocytes

• Published in: Neuroscience letters Vol. 685; pp. 173 - 178
• Main Authors: Taheri, Behnaz, Soleimani, Masoud, Aval, Sedigheh Fekri, Memari, Fatemeh, Zarghami, Nosratollah
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 15-10-2018
• Subjects: Astrocytes; Glial fibrillary acidic protein; Glioma; Matrix metalloproteinase; Microvesicles; Matrix metalloproteinase; Microvesicles; Glioma; Astrocytes; Glial fibrillary acidic protein
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2018.08.034

•C6 cell-derived microvesicles mainly ranged from 200 to 300 nm.•Activation of normal astrocytes with C6 microvesicles.•Upregulation of MMP2 and MMP14 in astrocytes incubated with C6MVs.•Downregulation of TIMP-2 in astrocytes incubated with C6MVs.
The interaction between glioma cells and the surrounding microenvironment plays a key role in tumor invasion and infiltration ability. Recent studies reported the importance of glioma-derived microvesicles in the interaction of the tumor and the surrounding environment. The purpose of this study was to scrutinize the role of glioma-derived microvesicles in the interaction between tumor and normal astrocytes, which are the most abundant non-neoplastic cells in the tumor microenvironment (TME). To this end, we examined the effect of C6 tumor cell-derived microvesicles in the activation of normal rat astrocytes. The results showed that exposing normal astrocytes to C6MVs increase the expression of the glial fibrillary acidic protein (GFAP), and activate normal astrocytes. In addition, incubation of normal astrocytes with C6MVs affects the expression of genes involved in tumor invasion and growth in these cells. Our findings suggest that C6 tumor cells through the secretion of microvesicles (MVs) can alter the phenotype of surrounding astrocytes as well as through the changes in the expression of the genes involved in extracellular matrix remodeling can predispose their invasion and growth.