Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 10; pp. 3964 - 3971
• Main Authors: Dong, Yiran, Sanford, Robert A, Boyanov, Maxim I, Kemner, Kenneth M, Flynn, Theodore M, O'Loughlin, Edward J, Locke, Randall A, Weber, Joseph R, Egan, Sheila M, Fouke, Bruce W
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01-10-2016
• Subjects: Bacillaceae - classification; Bacillaceae - genetics; Bacillaceae - isolation & purification; Bacterial Typing Techniques; Base Composition; BASIC BIOLOGICAL SCIENCES; Deep subsurface; DNA, Bacterial - genetics; Firmicutes; Groundwater - microbiology; Illinois; Iron - metabolism; Microbial iron reduction; Oxidation-Reduction; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Tepidibacillus
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001295

A Gram-stain-negative, microaerophilic rod-shaped organism designated as strain Z9T was isolated from groundwater of 1.7 km depth from the Mt. Simon Sandstone of the Illinois Basin, Illinois, USA. Cells of strain Z9T were rod shaped with dimensions of 0.3×(1-10) µm and stained Gram-negative. Strain Z9T grew within the temperature range 20-60 °C (optimum at 30-40 °C), between pH 5 and 8 (optimum 5.2-5.8) and under salt concentrations of 1-5 % (w/v) NaCl (optimum 2.5 % NaCl). In addition to growth by fermentation and nitrate reduction, this strain was able to reduce Fe(III), Mn(IV), Co(III) and Cr(VI) when H2 or organic carbon was available as the electron donor, but did not actively reduce oxidized sulfur compounds (e.g. sulfate, thiosulfate or S0). The G+C content of the DNA from strain Z9T was 36.1 mol%. Phylogenetic analysis of the 16S rRNA gene from strain Z9T showed that it belongs to the class Bacilli and shares 97 % sequence similarity with the only currently characterized member of the genus Tepidibacillus, T.fermentans. Based on the physiological distinctness and phylogenetic information, strain Z9T represents a novel species within the genus Tepidibacillus, for which the name Tepidibacillus decaturensis sp. nov. is proposed. The type strain is Z9T (=ATCC BAA-2644T=DSM 103037T).