Circulating microRNAs as emerging cardiac biomarkers responsive to acute exercise

• Published in: International journal of cardiology Vol. 264; pp. 130 - 136
• Main Authors: de Gonzalo-Calvo, David, Dávalos, Alberto, Fernández-Sanjurjo, Manuel, Amado-Rodríguez, Laura, Díaz-Coto, Susana, Tomás-Zapico, Cristina, Montero, Ana, García-González, Ángela, Llorente-Cortés, Vicenta, Heras, Maria Eugenia, Boraita Pérez, Araceli, Díaz-Martínez, Ángel E., Úbeda, Natalia, Iglesias-Gutiérrez, Eduardo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-08-2018
• Subjects: Adult; Biomarkers; Biomarkers - blood; Circulating MicroRNA - blood; Circulating MicroRNA - classification; Circulating microRNAs; Exercise; Exercise - physiology; Female; Healthy Volunteers; Heart disease; Heart Diseases - blood; Heart Diseases - diagnosis; Humans; Male; MicroRNAs - blood; Middle Aged; Myocardium - metabolism; Physical Endurance - physiology; Stress, Physiological; Time Factors; Biomarkers; Circulating microRNAs; Exercise; Heart disease
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/j.ijcard.2018.02.092

Circulating microRNAs (c-miRNAs) are mediators of intercellular communication with great potential as cardiac biomarkers. The analysis of c-miRNAs in response to physiological stress, such as exercise, would provide valuable information for clinical practice and a deeper understanding of the molecular response to physical activity. Here, we analysed for the first time the acute exercise response of c-miRNAs reported as biomarkers of cardiac disease in a well-characterized cohort of healthy active adults.
Blood samples were collected immediately before and after (0 h, 24 h, 72 h) a 10-km race, a half-marathon (HM) and a marathon (M). Serum RNA from 10-km and M samples was extracted and a panel of 74 miRNAs analysed using RT-qPCR. c-miRNA response was compared with a panel of nine cardiac biomarkers. Functional enrichment analysis was performed. Pre- and post-M echocardiographic analyses were carried out.
Serum levels of all cardiac biomarkers were upregulated in a dose-dependent manner in response to exercise, even in the absence of symptoms or signs of cardiac injury. A deregulation in the profiles of 5 and 19 c-miRNAs was observed for 10-km and M, respectively. Each race induced a specific qualitative and quantitative alteration of c-miRNAs implicated in cardiac adaptions. Supporting their discriminative potential, a number of c-miRNAs previously associated with cardiac disease were undetectable or stable in response to exercise. Conversely, “pseudo-disease” signatures were also observed.
c-miRNAs may be useful for the management of cardiac conditions in the context of acute aerobic exercise.
Circulating microRNAs could offer incremental diagnostic value to established and emerging cardiac biomarkers, such as hs-cTnT or NT-proBNP, in those patients with cardiac dysfunction symptoms after an acute bout of endurance exercise. Furthermore, circulating miRNAs could also show “pseudo-disease” signatures in response to acute exercise. Clinical practitioners should be aware of the impact caused by exercise in the interpretation of miRNA data.
•Different doses of acute exercise induce specific profiles of circulating microRNAs proposed as biomarkers of heart disease.•Circulating microRNAs offer incremental diagnostic value to other cardiac biomarkers in the context of acute exercise.•In absence of cardiac damage or dysfunction after acute exercise, circulating microRNAs show “pseudo-disease” signatures.•Our results suggest a potential role of circulating microRNAs as mediators of cardiac response to acute exercise.