Bovine serum albumin nanoparticles induce histopathological changes and inflammatory cell recruitment in the skin of treated mice

• Published in: Biomedicine & pharmacotherapy Vol. 107; pp. 1311 - 1317
• Main Authors: da Silva, Natalia Ingrid Oliveira, Salvador, Ezequiel Aparecido, Rodrigues Franco, Isabella, de Souza, Gabriel Augusto Pires, de Souza Morais, Stella Maria, Prado Rocha, Raissa, Dias Novaes, Rômulo, Paiva Corsetti, Patrícia, Malaquias, Luiz Cosme Cotta, Leomil Coelho, Luiz Felipe
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01-11-2018
• Subjects: Albumin; Animals; Cell Survival - drug effects; Drug Carriers - administration & dosage; Drug Carriers - toxicity; Female; Immunization; Injections, Subcutaneous; Mice; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - toxicity; Particle Size; RAW 264.7 Cells; Seroconversion; Serum Albumin, Bovine - administration & dosage; Serum Albumin, Bovine - toxicity; Skin - drug effects; Skin - immunology; Skin - pathology; Skin inflammation; Surface Properties; Vaccines - administration & dosage; Vaccines - immunology; Viral Nonstructural Proteins - administration & dosage; Viral Nonstructural Proteins - immunology; Nanoparticles; Albumin; Skin inflammation; Immunization
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2018.08.106

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•BSA-NPs are easily uptake by macrophagic RAW 264.7 and BHK-21 cells without any significant cytotoxicity.•Pro-inflammatory activity of BSA-NPs was showed using in vivo models.•BSA-NPs induce an inflammatory reaction in mouse skin after injection.•Mice immunized with rNS1 and BSA-NPs showed a high seroconversion rate if compared to animals immunized only with rNS1.
Albumin is a natural, biocompatible, biodegradable and nontoxic polymer and due to these features, nanoparticles made of albumin are a good system for drug or antigen delivery. Polymeric nanoparticles are being widely explored as new vaccines platforms due to the capacity of those nanoparticles to prime the immune system by providing sustained release of the antigen after injection. Biodegradable nanoparticles associated with proteins represent a promising method for in vivo delivery of vaccines. In our previous studies, bovine serum albumin nanoparticles (BSA-NPs) were identified as a promising system for in vivo delivery of microbial antigens. The aim of this work was to show the effect of BSA-NPs on skin after nanoparticles administration. The pro-inflammatory activity of BSA-NPs was evaluated using in vivo models. BSA-NPs are easily uptake by macrophagic RAW 264.7 and BHK-21 cells without any significant cytotoxicity. Histological examination of skin sections from BSA-NPs-treated mice revealed intense cellular infiltration, increased skin thickness, follicular hypertrophy, vascular congestion and marked collagenesis. Mice immunized with recombinant non-structural protein 1 (rNS1) from Dengue virus 1 and BSA-NPs showed a high seroconversion rate if compared to animals immunized only with rNS1. Therefore, the effect of BSA-NPs on skin after BSA-NPs administration has a biotechnological relevance to the rational design of vaccine formulations based on albumin nanocarriers. However in the next years future studies should be carried out to best characterize the effect of BSA-NPs on dendritic cells and establish the role of these nanoparticles as a new vaccine platform for infectious diseases or cancer.