Polymorphisms at the IL17A and IL17RA Genes are Associated with Prognosis of Papillary Thyroid Carcinoma

• Published in: Archives of medical research Vol. 53; no. 2; pp. 163 - 169
• Main Authors: Bertol, Bruna Cristina, Góes de Araújo, Jéssica Nayara, de Carvalho, Kleyton Thiago Costa, Gonzaga dos Santos, Marcos, Martelli-Palomino, Gustavo, de Figueiredo Feitosa, Nathalie Lobo, Maciel, Léa Maria Zanini, Silbiger, Vivian Nogueira, Lucena-Silva, Norma, Freitas, Janaina Cristiana de Oliveira Crispim, Donadi, Eduardo Antônio
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2022
• Subjects: Case-Control Studies; Genetic Predisposition to Disease; Genotype; Humans; IL-17A; IL-17RA; Interleukin-17 - genetics; Interleukin-17 - metabolism; Papillary thyroid carcinoma; Polymorphism, Single Nucleotide; Prognosis; Receptors, Interleukin-17 - genetics; Response to therapy; Single nucleotide polymorphisms (SNP); Thyroid cancer; Thyroid Cancer, Papillary - genetics; Thyroid Neoplasms - genetics; Single nucleotide polymorphisms (SNP); Response to therapy; Thyroid cancer; IL-17A; Papillary thyroid carcinoma; IL-17RA
• ISSN: 0188-4409; 1873-5487
• DOI: 10.1016/j.arcmed.2021.07.004

Interleukin (IL)-17A has a dual role in tumor immunity, promotes anti-tumor responses and facilitates angiogenesis by interacting with IL-17 receptor A (IL-17RA). Although IL-17A has been associated with the pathogenesis of papillary thyroid carcinoma (PTC), the nucleotide variability at the IL17A and IL17RA genes is still poorly characterized.
To assess the contribution of the IL17A (-197 G >A, rs2275913) and IL17RA (-947 A >G, rs4819554) single nucleotide polymorphisms (SNP) on the development and progression of PTC and on IL-17 plasma levels.
We studied 188 PTC patients and 170 healthy controls. SNPs were identified using PCR-amplified DNA and restriction fragment length polymorphism (RFLP) techniques. Plasma levels of IL-17A was evaluated in 83 PTC patients using ELISA. Statistical analyses were performed to evaluate the associations between SNPs and clinicohistopathological features of PTC and IL-17A levels.
No significant difference was observed regarding the allele and genotype distributions of both SNPs between PTC patients and controls. The IL17A GA was associated with poor biochemical and structural incomplete response to therapy, whereas no influence over the IL-17A expression was observed. The IL17RA AG was significantly associated with small-sized tumors, initial tumor stage at diagnosis and better response to therapy.
The IL17A SNP may predict an aggressive manifestation of PTC, whereas the IL17RA SNP was associated with a more favorable clinical outcome.