Trichoderma viride induces pathogenesis related defense response against rot pathogen infection in groundnut (Arachis hypogaea L.)

• Published in: Infection, genetics and evolution Vol. 34; pp. 314 - 325
• Main Authors: Gajera, H.P., Savaliya, Disha D., Patel, S.V., Golakiya, B.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-08-2015
• Subjects: Arachis - immunology; Arachis - microbiology; Aspergillus - physiology; Disease Resistance; Groundnut; Induced resistance; Microbial Interactions; Pathogenesis related enzymes; Phenylpropanoid enzymes; Plant Diseases - immunology; Plant Diseases - microbiology; Rot pathogen; Seeds - microbiology; Trichoderma - physiology; Rot pathogen; Pathogenesis related enzymes; Phenylpropanoid enzymes; Induced resistance; Groundnut
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2015.07.003

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•T. viride is the best bio-controller for reducing Aspergillus infection in groundnut.•Tolerant and susceptible varieties perform differently to combat the disease.•The specific activities of PPO and β-1,3 glucanase elevated at early on 3 DAS.•PAL induced in the T. viride JAU60 treated groundnut seeds at 6 DAS.•The chitinase activity sustained higher up to 9 DAS in tolerant varieties.•The elevation of defense enzymes by T. viride are different for groundnut varieties.
The study examine induction of defense enzymes involved in phenylpropanoid pathway and accumulation of pathogenesis related proteins in rot pathogen (Aspergillus niger Van Tieghem) challenged groundnut seedlings in response to Trichoderma viride JAU60. Seeds of five groundnut varieties differing in collar rot susceptibility were sown under non-infested, pathogen infested and pathogen+T. viride JAU60 seed treatment. Collar rot disease evident between 31.0% (J-11, GG-2) and 67.4% (GG-20) in different groundnut varieties under pathogen infested which was significantly reduced from 58.1% (J-11, GG-2) to 51.6% (GG-20) by Trichoderma treatment. The specific activities of polyphenol oxidase (EC 1.14.18.1) and β-1,3 glucanase (EC 3.2.1.6) elevated 3.5 and 2.3-fold, respectively, at 3days; phenylalanine ammonia lyase (EC 4.3.1.5) evident 1.6-fold higher at 6days; and chitinase (EC 3.2.1.14) sustained 2.3–2.8 folds up to 9days in Trichoderma treated+pathogen infested seedlings of tolerant varieties (J-11, GG-2) compared with moderate and susceptible (GAUG-10, GG-13, GG-20). T. viride JAU60 induces defense enzymes in a different way for tolerant and susceptible varieties to combat the disease. This study indicates the synergism activation of defense enzymes under the pathogenic conditions or induced resistance by T. viride JAU60 in a different groundnut varieties susceptible to collar rot disease.