Diversity and composition of microbial communities in Jinsha earthen site under different degree of deterioration

• Published in: Environmental research Vol. 242; p. 117675
• Main Authors: Li, Jing, He, Yanqiu, He, Changjie, Xiao, Lin, Wang, Ning, Jiang, Luman, Chen, Juncheng, Liu, Ke, Chen, Qiang, Gu, Yunfu, Ma, Menggen, Yu, Xiumei, Xiang, Quanju, Zhang, Lingzi, Yang, Tao, Penttinen, Petri, Zou, Likou, Zhao, Ke
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-02-2024
• Subjects: Alkalies; Bacteria - genetics; Bacteria and fungi; Deterioration; Earthen site; Function; Humans; Microbiota; Soil; Soil Microbiology; Function; Earthen site; Deterioration; Bacteria and fungi
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2023.117675

Earthen sites are the important cultural heritage that carriers of human civilization and contains abundant history information. Microorganisms are one of important factors causing the deterioration of cultural heritage. However, little attention has been paid to the role of biological factors on the deterioration of earthen sites at present. In this study, microbial communities of Jinsha earthen site soils with different deterioration types and degrees as well as related to environmental factors were analyzed. The results showed that the concentrations of Mg2+ and SO42− were higher in the severe deterioration degree soils than in the minor deterioration degree soils. The Chao1 richness and Shannon diversity indices of bacteria in different type deterioration were higher in the summer than in the winter; the Chao1 and Shannon indices of fungi were lower in the summer. The differences in bacterial and fungal communities were associated with differences in Na+, K+, Mg2+ and Ca2+ contents. Based on both the relative abundances in amplicon sequencing and isolated strains, the bacterial phyla Actinobacteria, Firmicutes and Proteobacteria, and the Ascomycota genera Aspergillus, Cladosporium and Penicillium were common in all soils. The OTUs enriched in the severe deterioration degree soils were mostly assigned to Actinobacteria and Proteobacteria, whereas the Firmicutes OTUs differentially abundant in the severe deterioration degree were all depleted. All bacterial isolates produced alkali, implying that the deterioration on Jinsha earthen site may be accelerated through alkali production. The fungal isolates included both alkali and acid producing strains. The fungi with strong ability to produce acid were mainly from the severe deterioration degree samples and were likely to contribute to the deterioration. Taken together, the interaction between soil microbial communities and environment may affect the soil deterioration, accelerate the deterioration process and threaten the long-term preservation of Jinsha earthen site.
•The microbial communities differed among deterioration types, degrees and seasons.•Mg2+ and SO42− contents were highest in the severe deterioration degree soils.•The differences in bacterial and fungal communities were associated with differences in Na+, K+, Mg2+ and Ca2+ contents.•Isolated bacteria and fungi produced acid or alkali that may accelerate deterioration.