The acoustic sensor for rapid analysis of bacterial cells in the conductive suspensions

• Published in: Ultrasonics Vol. 81; pp. 174 - 177
• Main Authors: Borodina, I.A., Zaitsev, B.D., Guliy, O., Teplykh, A.A., Shikhabudinov, A.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-11-2017
• Subjects: A phase and insertion loss of output signal; Biological acoustical sensor; Mini-antibodies; Suspension of bacterial cells; A phase and insertion loss of output signal; Suspension of bacterial cells; Mini-antibodies; Biological acoustical sensor
• ISSN: 0041-624X; 1874-9968
• DOI: 10.1016/j.ultras.2017.07.004

•Sensor for analysis of bacterial cells in the conductive suspensions is presented.•Sensor is based on two channel delay line on acoustic wave in piezoelectric plate.•The changes of output signals of sensor due to biological reactions were measured.•Sensor instantly responds on adding specific reagents to cell suspension.•Specific biological interaction increases the conductivity of cell suspension.
The possibility of using the acoustic sensor on the basis of a two-channel delay line for rapid analysis of bacterial cells in the conductive suspensions was investigated. The dependencies of change in phase and insertion loss of output signal of the sensor on conductivity of buffer solution with various concentrations of cells due to a specific interaction “bacterial cells - mini-antibodies” for electrically open and electrically shorted channels of delay line were measured. It has been found that these changes have the most values for the electrically open channel. It has been also shown that the sensor rapidly responds to the specific interaction and the time stabilization of the phase and insertion loss of output signal is less than 10min.