Breast milk immune composition varies during the transition stage of lactation: characterization of immunotypes in the MAMI cohort

• Published in: Frontiers in nutrition (Lausanne) Vol. 10; p. 1252815
• Main Authors: Rio-Aige, Karla, Fernández-Bargalló, Aina, Vegas-Lozano, Esteban, Miñarro-Alonso, Antonio, Castell, Margarida, Selma-Royo, Marta, Martínez-Costa, Cecilia, Rodríguez-Lagunas, Maria José, Collado, Maria Carmen, Pérez-Cano, Francisco José
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 2023
• Subjects: adipokines; breast milk; breastfeeding; cytokines; immunoglobulins; transitional stage; breast milk; adipokines; cytokines; breastfeeding; immunoglobulins; transitional stage
• ISSN: 2296-861X; 2296-861X
• DOI: 10.3389/fnut.2023.1252815

Breast milk is a complex and dynamic fluid needed for infant development and protection due to its content of bioactive factors such as immunoglobulins (Igs). Most studies focus primarily on IgA, but other types of Ig and even other immune components (cytokines and adipokines) may also play significant roles in neonatal health. As a first step, we aimed to characterize the Ig profile, many cytokines, and two adipokines (leptin and adiponectin) at two sampling time points within the transitional stage, which is the least studied phase in terms of these components. The secondary objective was to identify different breast milk immunotypes in the MAMI cohort substudy, and finally, we further aimed at analyzing maternal and infant characteristics to identify influencing factors of breast milk immune composition.
Breast milk samples from 75 mothers were studied between days 7 and 15 postpartum. The Igs, cytokines, and adipokine levels were determined by a multiplex approach, except for the IgA, IgM, and leptin that were evaluated by ELISA.
IgA, IgM, IgE, IgG2, IL-1β, IL-5, IL-6, IL-10, and IL-17 were significantly higher on day 7 with respect to day 15. The multiple factor analysis (MFA) allowed us to identify two maternal clusters (immunotypes) depending on the breast milk immune profile evolution from day 7 to day 15, mainly due to the IgE and IgG subtypes, but not for IgA and IgM, which always presented higher levels early in time.
All these results demonstrated the importance of the dynamics of the breast milk composition in terms of immune factors because even in the same lactation stage, a difference of 1 week has induced changes in the breast milk immune profile. Moreover, this immune profile does not evolve in the same way for all women. The dynamic compositional changes may be maternal-specific, as we observed differences in parity and exclusive breastfeeding between the two BM immunotype groups, which could potentially impact infant health.