Physio-biochemical basis of iron-sulfide nanoparticle induced growth and seed yield enhancement in B. juncea

• Published in: Plant physiology and biochemistry Vol. 118; pp. 274 - 284
• Main Authors: Rawat, Madhu, Nayan, Rajeev, Negi, Bhawana, Zaidi, M.G.H., Arora, Sandeep
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01-09-2017
• Subjects: Agronomic attributes; Brassica juncea; Iron Compounds - pharmacology; Iron sulfide nanoparticles; Mustard Plant - growth & development; Nanoparticles; Nutrient assimilation; Physio-biochemical basis; Seeds - growth & development; Sulfides - pharmacology; Nutrient assimilation; Agronomic attributes; Physio-biochemical basis; Iron sulfide nanoparticles; Brassica juncea
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2017.06.021

Metal nanoparticles have been reported to influence plant growth and productivity. However, the molecular mechanisms underlying the effects have not been completely understood yet. Current work describes the physio-biochemical basis of iron sulfide nanoparticle induced growth and yield enhancement in Brassica juncea. Iron sulfide nanoparticles (0, 2, 4, 6, 8 and 10 ppm) were used for foliar treatment of B. juncea at 30, 45 and 60 days after sowing, under field conditions. Foliar treatment of 4 ppm iron sulfide nanoparticle solution at 30 days after sowing brought maximal enhancement in agronomic attributes of the treated plants. Results of assays i.e. total chlorophyll, electrolyte leakage, Malondialdehyde (MDA), proline, H2O2 and antioxidant enzyme activities indicated the benign effect of iron sulfide nanoparticles on plants. Consequently, improved redox status of the treated plants, enabled them to assimilate higher photosynthate. The augmentation in growth and seed yield in iron sulfide nanoparticle treated plants was amply supported by activation of RUBISCO small subunit (rubisco S), RUBISCO large subunit (rubisco L), glutamine synthetase (gs) and glutamate synthase (gogat) genes. Thus, iron sulfide nanoparticle induced growth and yield enhancement is proposed to be mediated through activation of carbon and nitrogen assimilatory pathways at specific growth stage. The iron content in the leaves and root tissues of the treated plants was also significantly improved.
•NPs treatment improved growth, iron content, redox status and yield of B. juncea.•Induced expression of rubisco, gs and gogat lead to augmentation in growth & yield.•Right combination of NPs concentration and growth stage is critical for growth.