Bacillus subtilis cardiolipin protects its own membrane against surfactin-induced permeabilization

• Published in: Biochimica et biophysica acta. Biomembranes Vol. 1862; no. 10; p. 183405
• Main Authors: Pinkas, Dominik, Fišer, Radovan, Kozlík, Petr, Dolejšová, Tereza, Hryzáková, Klára, Konopásek, Ivo, Mikušová, Gabriela
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-10-2020
• Subjects: Bacillus subtilis; Bacillus subtilis - metabolism; Cardiolipin; Cardiolipins - metabolism; Cell Membrane - metabolism; Cell Membrane Permeability; Lipopeptides - metabolism; Liposome; Liposomes; Membrane; Peptides, Cyclic - metabolism; Surfactin; Liposome; Membrane; Surfactin; Cardiolipin; Bacillus subtilis
• ISSN: 0005-2736; 1879-2642
• DOI: 10.1016/j.bbamem.2020.183405

Surfactin, a cyclic lipoheptapeptide produced by Bacillus subtilis, is a surface-active antimicrobial that targets the barrier function of lipid membranes. It inserts itself into the membrane, where it forms conductive pores. Depending on its concentration, it eventually disintegrates the membrane in a detergent-like manner. The molecular details of this activity are not yet sufficiently understood, nor are the mechanisms that the surfactin producer employs to resist its own toxic product. We have previously shown that B. subtilis modifies its membrane lipid composition upon the onset of surfactin production, mainly increasing the cardiolipin content. Here we show that the increased cardiolipin content leads to a decreased surfactin-induced leakage of liposomes reconstituted from lipids isolated from the surfactin producer. This stabilizing effect of cardiolipin is concentration-dependent. Using a propidium iodide-based cell permeabilization assay, we further confirmed that the cytoplasmic membrane of the mutant B. subtilis strain lacking cardiolipin was substantially more susceptible to the action of surfactin, even though the amount of bound surfactin was the same as in the wild-type strain. We propose that membrane remodelling; due to the increase in cardiolipin content, contributes to the surfactin tolerance of B. subtilis.
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•Rate of surfactin-induced lysis is slower in membranes of surfactin-adapted cells.•Cardiolipin stabilizes the most the liposome integrity against surfactin.•The stabilizing effect of cardiolipin is concentration-dependent and linear.•CL-depleted B. subtilis cells are prone to surfactin-induced permeabilization.