Probiotic and immunostimulating effects of live and heat-killed Pediococcus pentosaceus TAP041

• Published in: Food science and biotechnology Vol. 33; no. 9; pp. 2189 - 2200
• Main Authors: Lee, Chaeeun, Park, Young-Seo
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01-07-2024; Springer Nature B.V; 한국식품과학회
• Subjects: Bacteria; Chemistry; Chemistry and Materials Science; Cyclooxygenase-2; Extracellular signal-regulated kinase; Food additives; Food Science; Functional foods & nutraceuticals; Heat; Immunostimulation; Kinases; Lysozyme; Macrophages; Nitric oxide; Nitric-oxide synthase; Nutrition; Pancreatin; Pediococcus pentosaceus; Phylogeny; Probiotics; Pyruvaldehyde; Reagents; Research Article; rRNA 16S; Transcription factors; 식품과학; Heat-killed probiotics; Probiotics; Immunostimulating activity; Pediococcus pentosaceus
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-024-01530-2

The selected strain, TAP041, showing an excellent ability to reduce the glyoxal and methylglyoxal levels, was identified by 16S rRNA gene-based phylogenetic analysis as
Pediococcus pentosaceus
. It demonstrated probiotic properties, including acid, bile salt, pancreatin, lysozyme tolerance, gut adhesion, and auto/coaggregation. In RAW264.7 macrophages, both live and heat-killed strains induced nitric oxide production and activated inducible nitric oxide synthase. RAW264.7 treated with
P. pentosaceus
TAP041 increased the expression level of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and cyclooxygenase-2, and regulated the expression of c-Jun amino-terminal kinase, p38, and extracellular signal-regulated kinase. These findings suggest that both live and heat-killed
P. pentosaceus
TAP041 can be used as potential immunostimulatory agents in functional food additives.